Mastodawn

Phages.fr Mar 15, 2023

Contrats to Cécile Breyton and collaborators !!
First structure of the complex between a phage RBP and its Receptor suggests a mechanism of how host recognition is transmitted to the phage

https://pubmed.ncbi.nlm.nih.gov/36779755/

@IBS_Grenoble
@INSB_CNRS
@Phages_fr

Deciphering Bacteriophage T5 Host Recognition Mechanism and Infection Trigger - PubMed

Bacteriophages, viruses infecting bacteria, recognize their host with high specificity, binding to either saccharide motifs or proteins of the cell wall of their host. In the majority of bacteriophages, this host recognition is performed by receptor binding proteins (RBPs) located at the extremity o …

PubMed

Phages.fr Mar 7, 2023

Great work showing thousands of novel phage satellites across hundreds of Bacterial spp by @doctor_jams @epcrocha
@jrpenades and @AlfredFillol
Includes software to find them.

https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkad123/7068371?login=false

Identification and characterization of thousands of bacteriophage satellites across bacteria

Abstract. Bacteriophage–bacteria interactions are affected by phage satellites, elements that exploit phages for transfer between bacteria. Satellites can encod

OUP Academic

Phages.fr Mar 1, 2023

Work led by Marianne De Paepe and PhD student Romain Sausset in collaboration with Luisa De Sordi on methods to count and characterise #phages and extracellular vesicles
Read it here👉http://bit.ly/3Y3Q2ia

@MYRIADELAB
@INRAE_Intl
@ScienceSorbonne
@crsa_paris

Phages.fr Feb 13, 2023

The 7th conference of the French Phage Network @Phages_fr
was held in October 2022 in Paris.
✨ A report summarizing all the brillant talks that were presented during this meeting is available here ✨
👉https://mdpi.com/1999-4915/15/2/495

📅Next conference in November 2023 in Lyon!

“French Phage Network” Annual Conference—Seventh Meeting Report

The French Phage Network (Phages.fr) has continuously grown since its foundation, eight years ago. The annual conference, held at the Institut Pasteur in Paris, attracted 164 participants from the 11th to the 13th of October 2022. Researchers from academic laboratories, hospitals and private companies shared their ongoing projects and breakthroughs in the very institute where Felix d’Hérelle developed phage therapy over a century ago. The conference was divided into four thematic sessions, each opened by a keynote lecture: “Interaction between phages, mobile genetic elements and bacterial immune system,” “Ecology and evolution of phage–bacteria interactions,” “Molecular interplay between phages and their hosts” and “Therapeutic and biotechnological applications of phages.” A total of 32 talks and 33 posters were presented during the conference.

MDPI

Phages.fr Feb 7, 2023

Phage production is blocked in the adherent-invasive Escherichia coli LF82 upon macrophage infection by P. Misson et al

https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011127

Phage production is blocked in the adherent-invasive Escherichia coli LF82 upon macrophage infection

Author summary Prophages are bacterial viruses stably integrated into their host, to which they can provide new functions, thus increasing their fitness in the environment. Thereby, they can participate to the virulence of bacterial pathogens. However, prophages are double-edged swords that can be awakened in response to genotoxic stresses, resulting in the death of their bacterial host. This raises the question of the effect of this type of stress in the natural environments where their bacterial hosts exert their virulence. In this study, we characterized the five active prophages present in Escherichia coli LF82, a strain belonging to the intestinal microbiota and suspected to be involved in Crohn’s disease via its ability to invade macrophages, a highly genotoxic environment. We show that LF82 inhibits the awakening of its prophages in macrophages, allowing it to survive there. Moreover, deletion of its most active prophage does not affect the viability of LF82 in this environment. These results suggest that LF82 has tamed its prophages in macrophages and also suggest that if they convey fitness advantages, they probably do so in environments differing from macrophages, and which remain to be discovered.

Phages.fr Jan 30, 2023

Nice review paper by Teagan Brown, Oliver Charity and @EvelienAdri
"Ecological and functional roles of bacteriophages in contrasting environments: marine, terrestrial and human gut"
https://www.sciencedirect.com/science/article/pii/S1369527422001138

Phages.fr Dec 14, 2022

#phage ecology paper
"Functional ecology of bacteriophages in the environment"
by Richard Puxty and Andrew Millard

Phages.fr Dec 5, 2022

"The host phylogeny determines viral infectivity and replication across Staphylococcus host species"
https://www.biorxiv.org/content/10.1101/2022.11.30.518513v1

by Sarah K Walsh, Ryan M Imrie, Marta Matuszewska, Gavin K Paterson, Lucy A Weinert, Jarrod D Hadfield, Angus Buckling and Ben Longdon

Phages.fr Dec 2, 2022

"Targeted suppression of human IBD-associated gut microbiota commensals by phage consortia for treatment of intestinal inflammation"

https://www.sciencedirect.com/science/article/abs/pii/S0092867422008509

Impressive article showcasing phage therapy in humans. Must read for #microbiome #microbiota #virome #phage

Phages.fr Nov 30, 2022

"Phage-antibiotic synergy: cell filamentation is a key driver of successful phage predation"
Authors: Julián Bulssico, Irina Papukashvili, Leon Espinosa, Sylvain Gandon, Mireille Ansaldi
(from @Phages_fr network!)

https://www.biorxiv.org/content/10.1101/2022.11.28.518157v1