MSCA Fellow postdoc at the Membrane Enzymology group, University of Groningen; tweeting about fluorescence microscopy, smFRET, membrane proteins, bacteria & GPCRs
https://scholar.google.nl/citations?user=lC_EQr4AAAAJ&hl=en
Single-molecule microscopy is full of surprises:
see how anisotropy can be used to follow the conformational dynamics! 🤯 #24-state-model
"Examination of conformational dynamics of AdiC transporter with fluorescence-polarization microscopy"
🔥🔬
Can antibiotics kill intracellular bacteria in vivo?
Researchers from Northeast Agricultural University, Harbin, China, developed safe and biocompatible nanopetides that penetrate host cells and kill intracellular bacteria by disrupting bacterial cell membranes and inducing excessive accumulation of ROS
Yongjie Zhu et al. (2025) in Science Advances: https://www.science.org/doi/10.1126/sciadv.ads3844
#review #microscopy #methods
"Advanced fluorescence lifetime-enhanced multiplexed nanoscopy of cells"
by Samrat Basak and RomanTsukanov
FLIM // super-resolution // SMLM // DNA-PAINT // multiplexed imaging // MIET
https://www.degruyter.com/document/doi/10.1515/mim-2024-0029/html
In this review paper, we summarize the significant advancements in the field of fluorescence lifetime imaging microscopy (FLIM), particularly wide-field FLIM with single-molecule sensitivity, achieved using the time-correlated single-photon counting-based position-sensitive LINCam system. Fluorescence lifetime adds valuable information beyond conventional intensity-based imaging, enabling diverse applications across research fields. Here, we focus on three primary bioimaging applications: (I) single-molecule FLIM in the far-red spectral region, (II) fast and multiplexed super-resolution imaging of cells, and (III) three-dimensional super-resolution imaging with high axial localization precision. Recent advances in position-sensitive detector technologies offer exciting opportunities for high-throughput super-resolution imaging with enhanced localization precision.
Dual-color 💚❤️ sub-millisecond single-molecule tracking via MINFLUX 🍩🔬
reveals concurrent diffusion of nAchR receptors 🔐 and fluorescent cholesterol
#preprint
https://www.researchsquare.com/article/rs-5619606/v1
Congrats to the authors! 👏👏
Nicotinic acetylcholine receptors (nAChRs) are ubiquitous neurotransmitter receptors predominantly located at the cell-surface of neurons and muscle cells. Their dynamics affect synaptogenesis at neurodevelopmental stages and the efficacy of synaptic transmission in the adult synapse. Here we ...
🚨 New insights into GPCR dynamics!
A simulation-driven mutagenesis study of A2A adenosine receptor shows how water molecules act as key facilitators of conformational change & receptor activity 💧🔬
By tweaking water-mediated contact networks, basal & ligand-induced activity can be predictably tuned
📄 Nature Chemistry: https://www.nature.com/articles/s41557-024-01719-2
Solvent-mediated interaction networks control a wide range of protein functions, but their design has been neglected owing to a lack of accurate computational tools. Now it is shown that allosteric signalling membrane proteins can be engineered through such networks, revealing a broader space of designable protein interactions and functions.
Kick off! Our first #GPCR ECI zoominar will take place on Jan. 26th. Tobias Benkel and Dylan S. Eiger @[email protected] will talk about their exciting stories on GPCR signaling. Be sure to come to the session a bit early for securing your place! @[email protected]
https://us06web.zoom.us/j/85397747413?pwd=NHFFZlk5NVV4bVVYbzMrc29hZVpTdz09#success
🐦🔗: https://twitter.com/GPCRzoominar/status/1615973508680499203
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We are excited to share the first pre-print that used an early prototype of our new microscope #Luminosa: "Super-resolved #FRET imaging by confocal fluorescence-lifetime single-molecule localization #microscopy". Read on arxiv: https://arxiv.org/abs/2211.15332
🐦🔗: https://twitter.com/PicoQuant/status/1615353549055197184
FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of molecular interactions and functional states. However, conventional FLIM and FRET imaging provide average information over an ensemble of molecules within a diffraction-limited volume, which limits the spatial information, accuracy, and dynamic range of the observed signals. Here, we demonstrate an approach to obtain super-resolved FRET imaging based on single-molecule localization microscopy using an early prototype of a commercial time-resolved confocal microscope. DNA Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) with fluorogenic probes provides a suitable combination of background reduction and blinking kinetics compatible with the scanning speed of usual confocal microscopes. A single laser is used to excite the donor, a broad detection band is employed to retrieve both donor and acceptor emission, and FRET events are detected from lifetime information.
Do you know that clathrin is involved in more than just endocytosis? 🧐 Our results suggest that 2D mesh-like clathrin structures at the plasma membrane are somehow linked to cancer metastasis.
Our latest manuscript is out on @[email protected]: http://dlvr.it/Sgr3Zt
Thread 👇🏻
🐦🔗: https://twitter.com/Rocha_Lab/status/1614912516949434368
Structural biologists have powerful metaphors to explain the importance of time-resolved methods and the structural dynamics of biomolecules. 🤓😎
The snapshots of a galloping horse are great, BUT
I will never use them again after I saw this image of a falling cat 🙏😍
🐈⬛🐈🐈⬛
Constant checking of your long-running data processing/simulations feeds your anxiety?
Use http://nfty.sh and get push notifications when the job is completed (or failed)!
Thanks to @[email protected] for the great idea👏