Brendan Innes

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Data scientist @PhenomicAI. Systems biology from scRNAseq. Days spent fussing over data viz. Dreams of bikes & mountains. He/him
innesbre.github.io
Fresh snow turns a Great Dane into Dobby.
#dogsofmastodon #dogstodon
Reminder: Transcript counts are not sufficient to distinguish doublets from #scRNAseq libraries. You can see this in the original DropSeq barnyard experiment, where libraries mapping to both mouse and human ("mixed") were used to determine the #doublet rate.
Transcript counts per cell are so broad that using a threshold to predict doublets is impossible. Yes, mean transcript count is higher in doublets, but there's huge overlap in the transcript count range between doublets and singlets.
New discovery: Nordic skiing is awesome, especially with family and the dog.