It’s a really good joke all around because the original sentence would be read as “asking all guys:” but the first response you read subverts that leading to the missing Oxford comma
10/10 would chuckle again
so it might genuinely be just a more inclusive form of the classic question
no, if this is what is meant then there is a grammatical error: “guys, tits, or ass?” would mean that, but “guys, tits or ass?” grammatically would mean, as i said, the question “ass or boobs” being asked to to “guys”.
oxford comma