Leszek@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
#academicChatter
Christian Meesters