Martin ReijnsDec 16, 2022
I'm super chuffed to have receive a Blue Flame Award for one of our plasmids distributed by @Addgene
#HappyDepositor
Show threadMartin ReijnsDec 16, 2022

We received the award for our most popular plasmid, pEGFP-RNASEH1 https://www.addgene.org/108699/

We made it as a control for our Nucleic Acids Research paper published in 2011 https://doi.org/10.1093/nar/gkq980

Addgene: pEGFP‐RNASEH1

Plasmid pEGFP‐RNASEH1 from Dr. Andrew Jackson's lab contains the insert RNASEH1 (nuclear isoform) and is published in Nucleic Acids Res. 2011 May;39(9):3652-66. doi: 10.1093/nar/gkq980. Epub 2011 Jan 17. This plasmid is available through Addgene.

Show threadMartin Reijns

pEGFP-RNASEH1 expresses the nuclear isoform of human RNase H1 with a C-terminal GFP tag

It seems to be most popular with labs working on #Rloops, because RNase H1 acts on RNA:DNA hybrids and overexpression can reduce R-loops

Dec 16, 2022 at 3:01pmWeb
Show threadMartin ReijnsDec 16, 2022
The plasmid was cloned by Katie Astell when she did her rotation project under my supervision in 2009, so it was great she visited from her postdoc lab @HuntLabEdin across town to have some cake with us to celebrate the award!