LeszekJul 8, 2025
First #cDNA @nanopore work I contributed to
Nano3P-seq: charting the coding and noncoding transcriptome at single-molecule resolution
#sequencing #poly_a_tail
https://www.nature.com/articles/s41596-025-01205-0
Nano3P-seq: charting the coding and noncoding transcriptome at single-molecule resolution - Nature Protocols

This protocol uses a customized nanopore cDNA sequencing method and a complementary software package, PolyTailor, to estimate the abundance and tail length and composition of multiple RNA biotypes at the single-molecule level.

Nature
CellBioNewsMar 11, 2024

How #cells manage their #mRNA stockpile and its output.

#embryo #maternal_stockpile #poly_A_tail

https://phys.org/news/2024-03-cells-mrna-stockpile-output.html

How cells manage their mRNA stockpile and its output

In a typical cell, genes encoded in DNA are used to make messenger RNA (mRNA), which is used to make proteins, and this process of gene expression keeps the cell running. Gene expression is regulated in each cell such that specific genes are turned on (making proteins) and their output is dialed up or down, driving the identity and behavior of each cell.

Phys.org