Conserved Regions of Resistance Genes as a Source of Nucleotide Polymorphisms in Wheat Hexaploid Research - Cytology and Genetics

Abstract The study was performed to identify primer pairs to conserved regions of R resistance genes to powdery mildew and other wheat pathogens effective in detecting polymorphism in amplicon spectra between samples contrasting in powdery mildew resistance. The resistant samples were the amphidiploid Aurotica (AAВВTT genome) and wheat lines developed on its basis (AAВВВDD). Detection of polymorphic components of spectra will make it possible to use appropriate primer pairs to assess the prospects of modern varieties of common wheat to be a recipient of the resistance gene(s) that can be transferred from Aurotica to the genetic pool of common wheat through sexual hybridization. The research method is PCR on genomic DNA of the studied genotypes using primer pairs developed using nucleotide sequences in conserved regions of powdery mildew resistance genes as well as degenerate primers to conservative regions of different resistance genes for arbitrary pairing of them using the RGAP method. The use of the RGAP method was shown to provide more information about the polymorphism present in the studied genomes compared to the use of primers to conserved sequences of Pm genes. They can be used in combinations with other RGAP primers to increase the number of effective PCR markers of resistance genes.