The protocol about MoP2 is out! MoP2 is a collection of @nextflowio pipelines for analysing Nanopore data with a special focus on dRNAseq. It can start from fast5 or fastq files and perform basecalling, demultiplexing, filtering, alignment, counting, assembling. https://link.springer.com/protocol/10.1007/978-1-0716-2962-8_13.
The results of the first pipeline can be fed to two other ones for polyA size estimation and modified base detection using different independent methods.
Nanopore Direct RNA Sequencing Data Processing and Analysis Using MasterOfPores
This chapter describes MasterOfPores v.2 (MoP2), an open-source suite of pipelines for processing and analyzing direct RNA Oxford Nanopore sequencing data. The MoP2 relies on the Nextflow DSL2 framework and Linux containers, thus enabling reproducible data analysis...
