Twelvetrees Lab

@[email protected]
18 Followers
12 Following
278 Posts
Axonal transport, one step at a time. Based at the University of Sheffield in the Faculty of Health. Most toots are automated and some are from @dozenoaks.
Websitehttps://www.twelvetreeslab.co.uk
ResearchCell & Molecular Neurobiology
LocationSheffield, UK
Twelvetrees LabJan 8, 2025
KIF1C activates and extends dynein movement through the FHF cargo adapter
https://www.nature.com/articles/s41594-024-01418-z
KIF1C activates and extends dynein movement through the FHF cargo adapter - Nature Structural & Molecular Biology

Here the authors show that codependence of dynein and kinesin KIF1C occurs through binding of the FTS–HOOK3–FHIP1B cargo adapter. Binding of KIF1C releases the HOOK3 autoinhibited folded conformation allowing dynein to bind the adapter. In this cocomplex, KIF1C further acts as a processivity factor for dynein.

Nature
Twelvetrees LabDec 21, 2024
Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo
http://biorxiv.org/cgi/content/short/2024.12.20.629623v1?rss=1
Twelvetrees LabDec 18, 2024
Axon-specific microtubule regulation drives asymmetric regeneration of sensory neuron axons [NEW RESULTS]
http://biorxiv.org/cgi/content/short/2024.10.10.617525v2?rss=1
Twelvetrees LabDec 18, 2024
Outcome-Driven Microscopy: Closed-Loop Optogenetic Control of Cell Biology [NEW RESULTS]
http://biorxiv.org/cgi/content/short/2024.12.12.628240v1?rss=1
Twelvetrees LabDec 11, 2024
Christmas has come early! A shiny new mass photometer from Refeyn - so much potential waiting to be unleashed ✨✨
Twelvetrees LabNov 19, 2024
Single Particle Tracking of Genetically Encoded Nanoparticles: Optimizing Expression for Cytoplasmic Diffusion Studies
http://biorxiv.org/cgi/content/short/2024.11.17.623896v1?rss=1
Twelvetrees LabOct 29, 2024
Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling
https://pubmed.ncbi.nlm.nih.gov/39466808/?utm_source=Inoreader&utm_medium=rss&utm_campaign=pubmed-2&utm_content=14o4TITEpscCsdP_6o21mcWPyHUEroxCC2gV88IyU4vVJnTQBB&fc=20230717091445&ff=20241028211342&v=2.18.0.post9+e462414
Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling - PubMed

To enable transmission of information in the brain, synaptic vesicles fuse to presynaptic membranes, liberating their content and exposing transiently a myriad of vesicular transmembrane proteins. However, versatile methods for quantifying the synaptic translocation of endogenous proteins during neu …

PubMed
Twelvetrees LabOct 24, 2024
Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons
https://pubmed.ncbi.nlm.nih.gov/39386502/?utm_source=Inoreader&utm_medium=rss&utm_campaign=pubmed-2&utm_content=14o4TITEpscCsdP_6o21mcWPyHUEroxCC2gV88IyU4vVJnTQBB&fc=20230717091445&ff=20241021125429&v=2.18.0.post9+e462414
Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons - PubMed

Plasma membrane protein degradation and recycling is regulated by the endolysosomal system, wherein endosomes bud from the plasma membrane into the cytosol and mature into degradative lysosomes. As such, the endolysosomal system plays a critical role in determining the abundance of proteins on the c …

PubMed
Twelvetrees LabJul 8, 2024
Paper alert: Rab6-Mediated Polarized Transport of Synaptic Vesicle Precursors Is Essential for the Establishment of Neuronal Polarity and Brain Formation
Read more here http://www.jneurosci.org/cgi/content/short/44/27/e2334232024?rss=1