Our September Issue is out! Image shows spinning disk confocal micrographs of an early C. elegans embryo expressing mScarlet-I labeled Plekstrin homology domain to visualize cell membranes. The two endoderm precursor cells are pseudocolored (yellow) and are shrinking their apical surfaces, resulting in internalization of these cells into the embryo over time.
From a study by Zhang et al. (https://doi.org/10.1083/jcb.202302102).
Read JCB’s September Issue: https://rupress.org/jcb/issue/222/9
Our July cover shows a maximum projection of GCaMP6s Ca2+-sensor signal from a 30-min recording of the epidermal basal stem cell layer in a live mouse. The color scale indicates GCaMP6s signal across time, showing a diversity of spatiotemporal signaling patterns. Local Ca2+ signaling is organized across the tissue in a global manner and is regulated by cell cycle and connexin 43.
From Moore, Bhaskar, Gao and colleagues (https://bit.ly/3pvKxNR).
Read JCB’s July issue: https://rupress.org/jcb/issue/222/7
Our June cover shows 4 cases of unilateral distribution of Cadm3 fused w/ GFP in WT donor retinal progenitor cells labeled w/ H2B-RFP in the p15+ zebrafish retina at 24 h post fertilization (hpf). Individual cases were imaged separately using an inverted laser-scanning confocal microscope and assembled. The retina structure on the background came from the 24-hpf Tg[zFucci] transgenic line injected with p15 mRNA. From Li et al. https://doi.org/10.1083/jcb.202204098
JCB’s June Issue: https://rupress.org/jcb/issue/222/6
Our May cover shows scanning electron microscopy micrograph of the specific adhesion between an HIV-1–infected T lymphocyte (colored in green) and a macrophage (colored in gray) before fusion of the two cells, leading to a very efficient macrophage infection by HIV-1.
From Mascarau et al. (https://doi.org/10.1083/jcb.202205103)
Read JCB’s May Issue: https://rupress.org/jcb/issue/222/5
Mascarau et al. demonstrate the relevance of HIV-1 infection of tissue-resident macrophages by fusion with infected CD4+ T cells and show that this process is m
Kozar-Gillan and colleagues reveal a novel molecular pathway in node of Ranvier formation by demonstrating that LGI3/2–ADAM23 interactions cluster and stabilize
Our March cover shows a maximum projection of Ashyba gossypii expressing the septin Cdc11a-GFP where color corresponds to the Z-height. Various septin structures can be observed in A. gosspyii: (1) interregion rings, which are bundled septin filaments that organize as bars; (2) basal collars that localize to lateral branches; and (3) thin filaments that run across hyphae.
From Cannon et al. (https://bit.ly/41xTh4n)
Read JCB’s March Issue: https://rupress.org/jcb/issue/222/3
Chii Jou Chan: The positives of being under "pressure"
Chii Jou (Joe) Chan (National University of Singapore) investigates how tissue mechanics and fluid pressure regulate mammalian development, with a special focus on folliculogenesis and oocyte quality control. For our #PeopleAndIdeas, we chatted with Joe about his scientific journey. https://rupress.org/jcb/article/222/2/e202211075/213830/Chii-Jou-Chan-The-positives-of-being-under?utm_source=mastodon
