Mastodawn

Leszek Aug 13, 2025

@nanopore DRS sequencing temperature was lowered to 30/31C (#P2 / #MinION) in #minknow v24.06 (and all recent versions) slowing down RNA helicase from 130 bps to 100-110 bps, lowering yield per hour and affecting signal readouts.
In effect, recent and older #RNA004 runs differ in base- and modification calling quality, possibly pA tail estimates.
This change was not widely announced to the community apart from a brief mention in software release notes!
https://community.nanoporetech.com/posts/software-release-24-06-for
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Leszek

Different helicase speed requires retraining all basecalling models. This change probably coincides with v5.0.0 models released with dorado v0.7.0
https://github.com/nanoporetech/dorado/releases/tag/v0.7.0
Because of that I suspect v5 models won't be optimal for basecalling older RNA004 runs sequenced at higher 35C / 130 bps (minknow older than v24.06, so before summer 2024).

Release v0.7.0 · nanoporetech/dorado

[0.7.0] (21 May 2024) This release of Dorado introduces new and more accurate v5 models for improved basecalling. It also adds a new subcommand, dorado correct, for single-read error correction to ...

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