Zach BennettCool new paper by the Lancaster group at Cornell. Very comprehensive stuff! Resonance Raman, EPR, stopped-flow. Amazing how one phenylalanine can be so crucial for the maturation and selectivity of the enzyme.
https://pubs.rsc.org/en/content/articlelanding/2023/sc/D3SC02288A
Outer coordination sphere influences on cofactor maturation and substrate oxidation by cytochrome P460
Product selectivity of ammonia oxidation by ammonia-oxidizing bacteria (AOB) is tightly controlled by metalloenzymes. Hydroxylamine oxidoreductase (HAO) is responsible for the oxidation of hydroxylamine (NH2OH) to nitric oxide (NO). The non-metabolic enzyme cytochrome (cyt) P460 also oxidizes NH2OH, but instead pro