Sven KlumpeGlad to finally share our manuscript on “Serial Lift-Out” by the amazing twitterless Oda Schiøtz, Jürgen Plitzko, @CJOKaiser , and myself. Doing multiple sections from single lift outs showing beautiful C. elegans ultrastructure 🤯 I could watch it all day 🤩How did we get there?
By using a copper adapter attached to the EasyLift needle, we have enabled lift-out of large volumes. We go on to deploy this on C. elegans L1 larvae frozen by a slightly adjusted method based on the amazing Waffle method @SEMC_NYSBC
This enabled us to extract HUGE chunks and obtain tons of lamellae from them. It revealed all the beautiful anatomy that the C. elegans L1 larva has to offer. We are only highlighting a few here: nerve ring, pharny, body wall muscle, hypodermis and many more!
We anticipate the method to enable the study of multicellular organisms and tissues by cryo-electron tomography. To do the proof-of-princple, take a look at this nice ribosome at 6.9 Å!
If we got you interested and you want to know more on how we sample molecular anatomy in whole organisms, check out the pre-print here: https://www.biorxiv.org/content/10.1101/2023.04.28.538734v1
It's been really stressful the last couple days, we locked ourselves in the seminar room finishing this story. After some nice celebration it is finally sleeptime😴
Thanks to all the co-authors @DustinMorado @jon___schneider @MatthiasPoge & twitterless Florian Beck + Christopher Thompson @thermosciEMSpec .
And we need to end with some cat content for my amazing, brilliant, and fascinating co-first authors Oda Schiøtz and @CJOKaiser and who are both proud cat parents (displayed is actually Christoph's cat 😁)
Thanks to all the co-authors @DustinMorado @jon___schneider @MatthiasPoge & twitterless Florian Beck + Christopher Thompson @thermosciEMSpec .