Martinho labFYI: if you are reading a manuscript from Portugal, beware that RT incubation typically means 16-18 celsius if the experiment was performed in the winter and 30-35 celsius in the summer :)
Mark Peifer@martinho_lab why we do all the fly work in a room set at 25
Albert Cardona@peiferlabunc @martinho_lab You are assuming labs are temperature controlled. In Barcelona, where I did my PhD, in June we could do the 37C antibody incubations on the bench. The AC was on but wasn't helping. Most experiments failed over June but somehow we never gave up, silly us.
Sarah Clatterbuck Soper@albertcardona @peiferlabunc @martinho_lab Even in the US with functional (and even purpose built supplementary!) AC my PhD lab gave up on RNA Native Gels in summer. I kind of suspected it was less about heat than humidity—the rigs were cold and water condensed on the gels as they ran.