A nice discussion on the "best human cell line" in the Quantixed blog: https://quantixed.org/2022/10/01/line-up-recommendations-for-the-best-human-cell-line/
#cellline #biology
Line Up: recommendations for the best human cell line

Here is a summary of the info I gleaned from asking for recommendations for the best human cell line. These were my criteria: For context, we currently use a number of human cell lines in the lab: …

quantixed
@cellosaurus U2OS is easy to grow and gorgeous to image but it seems like people don’t always take full account of its ALT status when using it for nuclear experiments. We don’t completely understand all the things implied by being ATRX null. DNA repair, chromatin state and nuclear foci are all going to be “weird” relative to a TERT cell.