Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody

This study provides a biochemical and biophysical analysis of Mannitou Fab, highlighting the intricate balance between stability, flexibility and function in engineered antibody fragments. The results underscore the critical role of glycosylation in modulating the oligomerization behaviour of Fab and preventing inappropriate intermolecular interactions, paving the way for more effective and reliable therapeutic antibodies in the future.