@chasewnelson

A classic. I still use it for simple things, MP and LS/NJ bootstrapping and distance matrix calculations. But it's more <2000, the last version was 2002 I think.

In 2006, we had already #MrBayes and #GARLI

And I already had gone #PhyloNetworks with SplitsTree in conjunction with PAUP* (for distance matrix), #RAxML (-III just out) and MrBayes.

Pics from:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2674679/

A Nuclear Ribosomal DNA Phylogeny of Acer Inferred with Maximum Likelihood, Splits Graphs, and Motif Analysis of 606 Sequences

The multi-copy internal transcribed spacer (ITS) region of nuclear ribosomal DNA is widely used to infer phylogenetic relationships among closely related taxa. Here we use maximum likelihood (ML) and splits graph analyses to extract phylogenetic information ...

PubMed Central (PMC)