π° "Keratin 7 immunohistochemistry reveals patterns of cell populations in liver biopsies from patients with MASLD"
https://doi.org/doi:10.1136/jcp-2025-210592https://pubmed.ncbi.nlm.nih.gov/41876217/ #Keratin
Keratin 7 immunohistochemistry reveals patterns of cell populations in liver biopsies from patients with MASLD
Aims Metabolic-associated steatotic liver disease (MASLD), which encompasses metabolic associated steatotic liver (MASL) and metabolic-associated steatohepatitis (MASH), is the most common chronic liver disorder worldwide. Fibrosis stage remains the most dependable histological predictor of prognosis; however, routine stains do not fully capture regenerative and injury-related processes. Keratin 7 (K7) immunohistochemistry highlights bile ducts (BDs), ductular reaction (DR), hepatic progenitor cells (PCs) and intermediate hepatocytes (IHs), all of which are involved in regeneration and fibrogenesis. This study aims to quantify K7-positive cell populations across histological stages of MASLD to enhance disease characterisation and evaluate the diagnostic value of K7 immunohistochemistry in this setting.
Methods Archived liver biopsies from a clinically well-characterised cohort of 36 patients (17 MASH, 19 MASL) were stained for K7, digitised and analysed using QuPath with manual expert review. K7-positive structures were classified into BD, DR, PC or IH, with over 10β000 objects quantified. Profile densities and portal tract normalised ratios were calculated and correlated with clinical, biochemical and histological grading and staging by the Steatosis Activity and Fibrosis score.
Results PC showed the strongest association with necro-inflammatory activity, peaking at grade 3. DR density increased significantly with fibrosis, supporting its role as an early marker of disease progression. IH was notably higher in MASH than in MASL, resulting in higher IH/DR ratios.
Conclusion Specific quantitation of K7-positive cell populations can refine the diagnosis of MASLD and complement routine scoring in clinical trials to improve patient stratification.
Data are available upon reasonable request.
π° "Voiding symptoms aggravate with decreasing stromal/epithelial ratio and increasing glandular-epithelial content in patients undergoing laser enucleation for benign prostatic hyperplasia, independently from prostate size"
https://doi.org/doi:10.1371/journal.pone.0345306https://pubmed.ncbi.nlm.nih.gov/41875110/ #Keratin
Voiding symptoms aggravate with decreasing stromal/epithelial ratio and increasing glandular-epithelial content in patients undergoing laser enucleation for benign prostatic hyperplasia, independently from prostate size
Introduction Benign prostatic hyperplasia (BPH) includes epithelial, stromal and mixed hyperplasia, but their specific contributions to voiding symptoms and prostate volume (PV) are unknown. Here, we examined relationships of symptoms and PV with stromal and epithelial markers in patients undergoing laser enucleation for BPH. Methods Tissues were obtained from holmium or thulium laser enucleation of the prostate (n = 146 patients). Expressions of the smooth muscle marker calponin-1 (CNN1) and the glandular-epithelial cell marker keratin-19 (KRT19) were assessed by RT-PCR and Western blot, and analyzed for correlation with international prostate symptom score (IPSS), maximum urinary flow rate (Qmax), and PV. Results The ratio of CNN1/KRT19 mRNA correlated positively with Qmax (r = 0.3809, p = 0.0263), and by trend negatively with IPSS (r = β0.2161, p = 0.0944). Accordingly, the IPSS increased with keratin protein expression (r = 0.4244, p = 0.0307), while the Qmax tended to correlate negatively with keratin expression (r = β0.2058, p = 0.4999). PV correlated negatively with CNN1 mRNA expression (r = β0.205, p = 0.0405). The inverse correlation of CNN1 with PV persisted in patients without catheterization (r = β0.2568, p = 0.0457), but was lacking in catheterized patients after separated analyses. Conclusions Voiding symptoms in patients undergoing laser enucleation for BPH aggravate with increasing keratin content. Symptoms in patients needing surgery for BPH depend rather on glandular-epithelial hyperplasia, but not on stromal hyperplasia, what might explain why these patients are refractory to treatment with Ξ±1-blockers.
π° "Green synthesis of Mohr's salt-modified keratin composite for selective removal of arsenate from polluted water"
https://doi.org/doi:10.1038/s41598-026-43190-8https://pubmed.ncbi.nlm.nih.gov/41872374/ #Keratinπ° "Bio-fabrication and 3D printing of keratin-enriched sodium alginate-gelatin scaffolds for the application of skin scar regeneration"
https://doi.org/doi:10.1177/09544119261427641https://pubmed.ncbi.nlm.nih.gov/41863140/ #Keratinπ° "Bio-fabrication and 3D printing of keratin-enriched sodium alginate-gelatin scaffolds for the application of skin scar regeneration"
https://doi.org/doi:10.1177/09544119261427641https://pubmed.ncbi.nlm.nih.gov/41863140/ #Mechanical #Keratinπ° "CircNOLC1/KRT16 Promotes Colorectal Cancer Invasion and Migration via c-Met Nuclear Translocation and Reprogramming of the Oxidative Pentose Phosphate Pathway"
https://doi.org/doi:10.1016/j.jbc.2026.111376https://pubmed.ncbi.nlm.nih.gov/41861993/ #Keratinπ° "UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8"
https://doi.org/doi:10.1016/j.tranon.2026.102728https://pubmed.ncbi.nlm.nih.gov/41861659/ #Keratinπ° "Heterologous expression of hagfish thread proteins EsTKΞ± and EsTKΞ³ in Pichia pastoris and in vitro self-assembly into an ordered fibrous network"
https://doi.org/doi:10.1016/j.synbio.2026.02.012https://pubmed.ncbi.nlm.nih.gov/41859730/ #Keratinπ° "Albumin-Keratin Casts Obstruct Renal Tubular and Vascular Lumens Following Kidney Ischemia"
https://doi.org/doi:10.1016/j.ekir.2026.106356https://pubmed.ncbi.nlm.nih.gov/41859375/ #Keratin
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