Glad to finally share our manuscript on “Serial Lift-Out” by the amazing twitterless Oda Schiøtz, Jürgen Plitzko, @CJOKaiser , and myself. Doing multiple sections from single lift outs showing beautiful C. elegans ultrastructure 🤯 I could watch it all day 🤩How did we get there?
Sven Klumpe
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Member of the Plitzko Lab at MPI for Biochemistry combining CryoEM/ET with Computational Biophysics/Biochemistry! Pushing CryoET into DevBio and tissues. he/him
And we need to end with some cat content for my amazing, brilliant, and fascinating co-first authors Oda Schiøtz and @CJOKaiser and who are both proud cat parents (displayed is actually Christoph's cat 😁)
Thanks to all the co-authors @DustinMorado @jon___schneider @MatthiasPoge & twitterless Florian Beck + Christopher Thompson @thermosciEMSpec .
Thanks to all the co-authors @DustinMorado @jon___schneider @MatthiasPoge & twitterless Florian Beck + Christopher Thompson @thermosciEMSpec .
It's been really stressful the last couple days, we locked ourselves in the seminar room finishing this story. After some nice celebration it is finally sleeptime😴
We anticipate the method to enable the study of multicellular organisms and tissues by cryo-electron tomography. To do the proof-of-princple, take a look at this nice ribosome at 6.9 Å!
This enabled us to extract HUGE chunks and obtain tons of lamellae from them. It revealed all the beautiful anatomy that the C. elegans L1 larva has to offer. We are only highlighting a few here: nerve ring, pharny, body wall muscle, hypodermis and many more!
By using a copper adapter attached to the EasyLift needle, we have enabled lift-out of large volumes. We go on to deploy this on C. elegans L1 larvae frozen by a slightly adjusted method based on the amazing Waffle method @SEMC_NYSBC
Drosophilst probably know this already but I just digged out and fell in love with this beauty 🤩, the first recorded description of #Drosophila: Johann Wilhelm Meigen (1830) “Systematische Beschreibung der bekannten europäischen zweiflügeligen Insekten”(https://www.biodiversitylibrary.org/item/132636#page/430/mode/1up)
Glad that the participants of #EMBOinsitu voted for me to get this prize! What directly pops into the head though: Does democratic voting of poster prizes feed into gender bias? Maybe worthwhile to collect data on @EMBLEvents and maybe adjust policy accordingly ? :)
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RT @EMBLEvents
Check out the best posters from 'In situ structural biology: from cryo-EM to multi-scale modelling'. 👀Congratulations to our five poster prize winners Joe…
https://twitter.com/EMBLEvents/status/1634185996752281603
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RT @EMBLEvents
Check out the best posters from 'In situ structural biology: from cryo-EM to multi-scale modelling'. 👀Congratulations to our five poster prize winners Joe…
https://twitter.com/EMBLEvents/status/1634185996752281603