Multiphoton imaging through 20 mm of air with the new Cousa objective.

This is a new type of objective: ultra long working distance, huge field-of-view, optimized for two-photon imaging.

And it fits on standard microscopes neuroscientists use for in vivo imaging (e.g., from Bruker, Thorlabs, Neurolabware, INSS, CoSys, etc.)!

The Cousa provides excellent data and even three-photon imaging over a huge field-of-view!

The paper has data from marmosets, tree shrews, both young and adult ferrets, intact pig eye, the first in vivo calcium imaging from mouse cochlear hair cells (!), beautiful input mapping / dendritic spine imaging, and more!

The paper was published just now: https://doi.org/10.1038/s41592-023-02098-1 It's open access. The Cousa objective can be purchased from a company that is being spun out of the lab: https://pacificoptica.com

We've all been there: it's puzzle time, but once you dump out the pieces and start laying them flat, you realize you don't have enough space on your table. Join me as we use physics to find out ✨HOW BIG A TABLE YOU NEED FOR YOUR JIGSAW PUZZLE ✨

https://doi.org/10.48550/arXiv.2312.04588
#SciComm

How long-read sequencing will transform neuroscience.

By Tychele Turner

https://www.thetransmitter.org/whole-genome-sequencing/how-long-read-sequencing-will-transform-neuroscience/?utm_source=mastodon&utm_medium=org-social&utm_campaign=20231122-how-long-read-sequencing-will-transform-neuroscience

If you're seeing dire news about the water & alfalfa lately in the US, I have good news for you.

The US's water problems have solutions!

One of the most powerful solutions: give serious attention & investment to agriculture in the southeastern US.

Let's talk about just one way to do that.

We have a couple of peer reviewer training resources available on our website: https://plos.org/resource/peer-review-training-resources/

Happy to answer questions about this.

I also regularly ask postdocs and senior lab members to review manuscripts but can do that only if I find can find information about your research area and expertise online. If you are interested in reviewing neuroscience manuscripts for PLOS Biology, let me know. Happy to work with you if the right manuscripts for your expertise comes along.

Hi #infosec community: any recommended practices for preventing a personal blog webpage from being dominated by crawlers?

The robots.txt seems largely ignored. Crawlers presently consume ~75% of resources according to the access logs.

I don't mind not being listed on any web search engines.

Would a basic auth work? Can one make the basic auth dialog show a message that provides the password hint in it, like "What is my first name?"

#blogging

Nadine Randel and I wrote a dispatch on the recent miniature-wasp eye #connectome paper
Have a look:
https://www.sciencedirect.com/science/article/pii/S096098222301432X?dgcid=author

and here is the original paper on the Megaphragma eye by Chua and colleagues https://www.sciencedirect.com/science/article/pii/S096098222301237X

#neuroscience #microscopy