| Website | https://www.gokcelab.com |
| @ozgungokce |
RT @AlexYermanos
Sharing our new publication in Acta Neuropathologica investigating the selection of virus-specific, autoreactive B cells and local tolerance in the central nervous system! Fun collab w/ @ReddyLab_ETHZ @LabOxenius @merkler_lab @edubeltran_lab et al
https://link.springer.com/article/10.1007/s00401-023-02537-5
B cells contribute to the pathogenesis of both cellular- and humoral-mediated central nervous system (CNS) inflammatory diseases through a variety of mechanisms. In such conditions, B cells may enter the CNS parenchyma and contribute to local tissue destruction. It remains unexplored, however, how infection and autoimmunity drive transcriptional phenotypes, repertoire features, and antibody functionality. Here, we profiled B cells from the CNS of murine models of intracranial (i.c.) viral infections and autoimmunity. We identified a population of clonally expanded, antibody-secreting cells (ASCs) that had undergone class-switch recombination and extensive somatic hypermutation following i.c. infection with attenuated lymphocytic choriomeningitis virus (rLCMV). Recombinant expression and characterisation of these antibodies revealed specificity to viral antigens (LCMV glycoprotein GP), correlating with ASC persistence in the brain weeks after resolved infection. Furthermore, these virus-specific ASCs upregulated proliferation and expansion programs in response to the conditional and transient induction of the LCMV GP as a neo-self antigen by astrocytes. This class-switched, clonally expanded, and mutated population persisted and was even more pronounced when peripheral B cells were depleted prior to autoantigen induction in the CNS. In contrast, the most expanded B cell clones in mice with persistent expression of LCMV GP in the CNS did not exhibit neo-self antigen specificity, potentially a consequence of local tolerance induction. Finally, a comparable population of clonally expanded, class-switched, and proliferating ASCs was detected in the cerebrospinal fluid of relapsing multiple sclerosis (RMS) patients. Taken together, our findings support the existence of B cells that populate the CNS and are capable of responding to locally encountered autoantigens.
Yapici lab 🖨️🔥 off the press!!
The story of novel visual feedback neurons make sure that males flies court nice n’ steady 😎
Kudos to the awe-inspiring twitterless first-author Yuta, and Nilay (also twitterless now!) & to @XinyueCui , @HaeinKim6🎉🎉
My first post on Mastodon after a whole lot of lurking and boosting, so I'm treating this as a mini #introduction for myself
I'm an #immunology PhD candidate at #ColumbiaUniversity medical center, and we just published a study of #TissueSpecifity of human #Tcell identity and clonal expansion in barrier sites, using #CyTOF, #scRNAseq, and #TCR #sequencing!
Farber and colleagues examine the phenotypic, transcriptomic, clonal, and functional differences between tissue-resident T cells in various barrier tissue sites relative to T cells in lymphoid organs and circulation in humans.
#migration#re-Introduction.
I'm a theoretical neuroscientist at U Mainz Medical Center and co-affiliated with U Bonn Medical center. Primary focus on cortical circuits, their network activity, synaptic plasticity and protein dynamics in dendrites. Broadly interested how circuits learn e.g. neuro/AI interface and want to understand how neural networks compute, both algorithmically and intracellularly. Occational posts about societal and academic issues.
RESEARCH NEWS: New stats test just published from @[email protected] for distinguishing biological variation from technical variation in #singlecell datasets. See the thread for links to a tutorial, scripts and visualisation tools.
🧵https://twitter.com/LabListon/status/1615673972850413569
🐦🔗: https://twitter.com/BabrahamInst/status/1616042920284389377
“We have an exciting #computational paper out in @CellRepMethods. Ever use a #tSNE or #UMAP in #scSeq, #flowcytometry or #masscytometry? It doesn't have to be just a pretty picture anymore - we've developed a statistical test to check for differences. 1/6 https://t.co/rV27mf52Ub”
Tatiana Segura
Gaspar Jekely
Joe Sabatino
Deepthi Mahishi
Daniel Caron
ttchumatchenko
Adrian Liston