Do you (want to) use whole-plasmid sequencing (e.g. @plasmidsaurus) but are put off by $15/sample? Use SAVEMONEY—our (postdoc Masaaki Uematsu’s) algorithm that lets you mix 6 or more plasmids/sample & computationally de-mixes reads!

https://colab.research.google.com/github/MasaakiU/MultiplexNanopore/blob/master/colab/MultiplexNanopore.ipynb

https://www.biorxiv.org/content/10.1101/2023.04.12.536413v1

How it works: 1/n

Thrilled to share this story in JACS Au! https://pubs.acs.org/doi/10.1021/jacsau.2c00419

Takeaways:
1) analogs of PC lipids w/different choline structures adopt different organelle localizations & are useful for tracing interorganelle lipid transport
2) OCT1, a liver transporter typically used to study PK/drug efflux, can also facilitate uptake of small cationic #ChemicalBiology probes, including #bioorthogonal choline analogs. Available soon @Addgene https://addgene.org/188995/

Congrats, Dylan!
#lipidtime #OpenAccess