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FocalPlane is a community site for anyone with an interest in microscopy.
FocalPlanehttps://focalplane.biologists.com/

This #TechnicalTuesday we’re reading the #preLight from Girish Kale highlighting the beautiful #preprint on embryo mechanics from Sacha Ichbiah, Hervé Turlier, & team.

@preLights https://prelights.biologists.com/highlights/embryo-mechanics-cartography-inference-of-3d-force-atlases-from-fluorescence-microscopy/

Embryo mechanics cartography: inference of 3D force atlases from fluorescence microscopy - preLights

May the (3D map of the) force be with you, and guide you through the labyrinth of embryonic development

preLights

The deadline for Workshop proposals with @Co_Biologists is fast approaching. We’re looking for proposals that address cutting-edge science on emerging themes.

Deadline: 30 June 2023

Check out our post to find out more: https://focalplane.biologists.com/2023/06/12/the-company-of-biologists-workshops-2025/

The Company of Biologists Workshops 2025 - FocalPlane

The Company of Biologists Workshops 2025 - News

FocalPlane

This #EMmonday we are focussing on SBF-SEM with a case study from Federica Mangione. Federica and colleagues used SBF-SEM to dissect the cellular assembly of tactile organ of Drosophila, identifying a new cell type, the F-Cell.

https://focalplane.biologists.com/2023/05/19/inputs-and-outputs-of-vem-in-a-sensory-system/

#volumeEM #drosophila #devbio

For more from the volume EM community: https://focalplane.biologists.com/category/volume-em/

Inputs and Outputs of vEM in a Sensory System - FocalPlane

Inputs and Outputs of vEM in a Sensory System - Volume EM

FocalPlane

We’re celebrating #FluorescenceFriday by highlighting the #OpenAcess @BiologyOpen study from Siyu Yang, Mingzhe Cao and colleagues. They use #zebrafish to investigate retinal development and function and find that loss of flrt2 leads to microphthalmia.

https://journals.biologists.com/bio/article/12/6/bio059784/316746/Loss-of-flrt2-gene-leads-to-microphthalmia-in

Loss of flrt2 gene leads to microphthalmia in zebrafish

Summary: flrt2 is necessary for the normal development of the zebrafish eyes since it is strongly expressed in the retina and deletion of flrt2 causes microphthalmia in zebrafish

The Company of Biologists

📢 New post on FocalPlane

Next up in our rotation of preprints topics are new tools and techniques in imaging. The list this week includes advances in hardware, sample preparation, probes and accessible imaging systems. Let us know if we have missed any recent preprints from your reading list. https://focalplane.biologists.com/2023/06/16/microscopy-preprints-new-tool-and-techniques-in-imaging-5/

Microscopy preprints – new tool and techniques in imaging - FocalPlane

Microscopy preprints – new tool and techniques in imaging - Discussions

FocalPlane
🌟 Deadline incoming for our 2023 Image competition!
Get your images to us by Monday 19 June for your chance to see your image on the cover of @J_Cell_Sci and win £100. Thanks to everyone that has already submitted image, we can’t wait to share them!
https://focalplane.biologists.com/2023/05/15/focalplane-image-competition-2023/
FocalPlane image competition 2023 - FocalPlane

FocalPlane image competition 2023 - Default

FocalPlane

📢 New post on FocalPlane

In our ‘Community collective’ posts we aim to give a permanent (and searchable) home for those nuggets of advice collected from the community. Our first post focusses on 3D viewers after a request for software recommendations from @engineeringjoy

https://focalplane.biologists.com/2023/06/14/community-collective-viewing-z-stacks-in-3d/

If you have requested (or seen) any microscopy advice that you would like to see be given a home on FocalPlane, comment here or contact us at [email protected]

#microscopy
#cellbiology

Community collective: viewing z-stacks in 3D - FocalPlane

Community collective: viewing z-stacks in 3D - Discussions

FocalPlane

Our aMAZ(E)ing #MidweekMovie comes from Connie Shen, Judith Mandl, Nienke Vrisekoop and colleagues. They discover that neutrophils with segmented nuclei have a speed advantage when migrating through confinement spaces

📖 @J_Cell_Sci: https://journals.biologists.com/jcs/article/136/11/jcs260768/316606/Nuclear-segmentation-facilitates-neutrophil

Nuclear segmentation facilitates neutrophil migration

Highlighted Article: Using heterogenous neutrophils mobilized following endotoxin administration in humans, we show that neutrophils with nuclei segmented into more lobes can migrate faster in a physically confined space.

The Company of Biologists
This #TechnicalTuesday we are highlighting a FocalPlane post from Mara Lampert on tracking in napari. Mara describes the different tracking options, guides the user through the process & calls for feedback on the tools.
https://focalplane.biologists.com/2023/06/01/tracking-in-napari/
You can find Mara’s series, ‘Bio-image Analysis with Napari here: https://focalplane.biologists.com/2023/06/01/tracking-in-napari/
Tracking in napari - FocalPlane

Tracking in napari - Default

FocalPlane

This #EMmonday we are looking at these beautiful L. mexicana axoneme cross-sections from Chandra Ramakrishnan, Cécile Fort, Linda Kohl, Richard Wheeler, Robert Sinden and colleagues. They examined the role of Rsp9 in axoneme assembly.
#OpenAccess

https://journals.biologists.com/jcs/article/136/11/jcs260655/316601/Radial-spoke-protein-9-is-necessary-for-axoneme

Radial spoke protein 9 is necessary for axoneme assembly in Plasmodium but not in trypanosomatid parasites

Summary: Radial spoke head protein 9 (RSP9) is found in the flagella of trypanosomatids and malaria parasites, and is important for motility in trypanosomatids but critical for fertilisation in Plasmodium.

The Company of Biologists