RahelWoldeyes

45 Followers
95 Following
21 Posts
A structural cellular Biologist #teamtomo #cryoem
Visiting Faculty Program (VFP) H... | U.S. DOE Office of Science (SC)

The Visiting Faculty Program is sponsored and managed by the DOE Office of Science’s Office of Workforce Development for Teachers and Scientists (WDTS) in collaboration with the DOE laboratories.

“Molecular architecture of the actin cytoskeleton: From single cells to whole organisms using cryo-electron tomography” [https://doi.org/10.1016/j.ceb.2024.102356] by Jonathan Schneider and Marion Jasnin. Thank you for citing our preprint! #goodread #preprintsadvancescience
5/5 A big thank you to our collaborators and #teamtomo!
4/5 Found that the troponin complex adopts a variety of conformations in different conditions
3/5 Reconstructed sub-nanometer resolution in situ structures of the human cardiac thin filament
2/5 Visualized subcellular arrangement of macromolecules in cardiomyocytes
Very excited to share our paper. https://www.biorxiv.org/content/10.1101/2023.10.26.564098v1
We combine cutting-edge developments in cryo-electron tomography with hiPSC-cardiomyocytes to visualize cardiac proteins in their cellular context in health and disease. #cryoem #teamtomo #cryoet
🧵 1/5

Cool article about the current state of #cryoET #teamTOMO

Catching proteins at play: the method revealing the cell’s inner mysteries
https://www.nature.com/articles/d41586-023-02909-7

Catching proteins at play: the method revealing the cell’s inner mysteries

Cryo-electron tomography is a hugely promising tool in visual proteomics — if researchers can work out what they are seeing.

We directly visualized the selective enrichment of actin at presynapses, and could get good SMLM images of presynaptic actin at the nanoscale within bead-induced presynapses 5/23
See our new preprint on time-resolved cryo-EM of biological molecules:
biorxiv.org/content/10.1101/2023.01.25.525430v2.full.pdf