Basically the Legion from the Bible is what plural trans women were doing before transition and anonymous imageboards were invented.
Aspirantka in exile. Villainous toolmaker.
| Pronouns and grammar | Вы/ж.р. |
| ORCID | 0009-0001-0217-1650 |
Iris
@Iris_IllEra
- 31 Followers
- 14 Following
- 989 Posts
Plant physiology. Accessible material science. Molecular biology of model objects.
Aspirantka in exile. Villainous toolmaker.
Aspirantka in exile. Villainous toolmaker.
Cassandra is only carbon now"WPS AI revolutionizes your workflow by offering functions like AI-Powered document scanner, AI-generated content, rewriting, ChatPDFs, and more."
I am now less intelligent than I was before I read that. A part of my soul has gone on permanent vacation, leaving me high and dry.
bioRxiv BiochemistryAdvancing Therapeutic Solutions: Poloxamer-based Thermosensitive Injectable Hydrogels containing a Self-assembling Peptide for In situ Gelation in an Osteoarthritis Murine Model https://www.biorxiv.org/content/10.1101/2025.04.30.651282v1?med=mas
Advancing Therapeutic Solutions: Poloxamer-based Thermosensitive Injectable Hydrogels containing a Self-assembling Peptide for In situ Gelation in an Osteoarthritis Murine Model
This study presents the development and characterization of a novel thermosensitive injectable hydrogel designed to enhance the biomechanical properties of poloxamer 407 (P407) through the incorporation of a self-assembling peptide. The primary objective was to engineer a formulation that rapidly gels following intra-articular (i.a.) injection, exhibits improved mechanical strength, and enables sustained release of embedded therapeutic cargo. Gelation time assays demonstrated that the P407-peptide formulation solidified more quickly than P407 alone at equivalent concentrations. Rheological analysis revealed a 1.5kPa increase in storage modulus in the hybrid hydrogel, confirming improved mechanical integrity. In vitro biocompatibility was assessed using human chondrocytes, with MTS assays and LIVE/DEAD staining indicating no cytotoxicity across tested concentrations. To evaluate in vivo applicability, a near-infrared fluorescent (NIRF) dye was incorporated into the hydrogel and injected intra-articularly into an osteoarthritis (OA) mouse model. The labeled formulation allowed for successful tracking and demonstrated localized gelation, supporting its suitability for site-specific, sustained delivery. Overall, the P407-peptide hydrogel offers a promising platform for i.a. therapeutic applications, combining injectability, rapid thermoresponsive gelation, mechanical reinforcement, and controlled release behavior, making it well-suited for regenerative medicine and OA treatment. ### Competing Interest Statement The authors have declared no competing interest.
rosa ⚬.⚬i love journal names. cant wait to publish my paper in Concept or Device
or most recently
[ Blood ]
elena (⧞)went into a second hand shop in kobe today, got to play the 'does this globe have the soviet union on it' game with my buddy and then we both went 'IT DOES'
b-rain
A mutation at the dimer interface regulates catalysis https://www.biorxiv.org/content/10.1101/2025.04.29.650001v1?med=mas
A mutation at the dimer interface regulates catalysis
Fluoroacetate Dehalogenase (FAcD), a homodimeric dehalogenase of interest in bioremediation, displays half-of-the-sites reactivity, requiring allosteric communication between the subunits to coordinate the reaction. Dimer interfaces are an obvious site of interest for understanding communication between subunits and have been shown, in a variety of enzyme systems, to mediate such communication. However, mutations at these interfaces often need to be substantial to noticeably affect protein activity. In this study, we demonstrate that two subtle interface mutations of FAcD, either accelerate the reaction or results in near-complete loss of activity with structural evidence of disruption at the dimer interface. By examining these variants using ultrahigh-resolution crystallography and kinetics studies, the influence of dimer interface variations in hydrogen-bonding networks on enzyme activity can be elucidated. ### Competing Interest Statement The authors have declared no competing interest. Deutsche Forschungsgemeinschaft, https://ror.org/018mejw64, 451079909
Massively parallel characterization of RNA G-quadruplex stability and molecular recognition https://www.biorxiv.org/content/10.1101/2025.04.29.651304v1?med=mas
Massively parallel characterization of RNA G-quadruplex stability and molecular recognition
RNA G-quadruplexes (rG4s) have been implicated as important regulators of RNA metabolism and are promising targets for RNA-targeted therapeutics. rG4s typically require a canonical (G≥2N1-7)4 motif, but the sequence features that affect rG4 stability and recognition by RNA-binding proteins (RBPs) and rG4-binding ligands are not fully understood. To interrogate sequence-level drivers of rG4 folding, we applied a reverse-transcriptase stop sequencing strategy to a library of ~3,000 synthetic rG4s with varied G-tract lengths, loop lengths, and loop compositions, permitting massively parallel quantification of rG4 stability. Our data confirm known sequence-level features and characterize novel combinatorial impacts of these features. We also assessed systematically mutagenized natural rG4s, revealing unexpected mutations that significantly affect rG4 stability, including contributions from flanking sequences outside of the rG4. We further used our strategy to assess rG4 recognition preferences of the model rG4-ligand pyridostatin, revealing a preferential stabilization of rG4s containing mixed-length G-tracts. We further demonstrated the potential for large-scale protein binding assays with our library to reveal rG4 features recognized by RBPs, specifically G3BP1 and FMRP. Our approach and data provide a generalizable framework to study sequence-level drivers of rG4 stability, binding by RBPs, and ligand interactions, defining basic principles of rG4 formation and downstream biology. ### Competing Interest Statement The authors have declared no competing interest. National Institute of General Medical Sciences, https://ror.org/04q48ey07, R35GM142864, R35GM147010, T32GM135095 University of North Carolina at Chapel Hill, https://ror.org/0130frc33, National Heart, Lung, and Blood Institute (NHLBI), , T32HL007149 UNC Simmons Scholar Award, ,
Entering the fight against suicidal thoughts on the side of suicidal thoughts and losing
vera grey 
solidarity catgirl wants you to join a unyan