Local negative feedback of Rac activity at the leading edge underlies a pilot pseudopod-like program for amoeboid cell guidance

To migrate efficiently, neutrophils must polarize their cytoskeletal regulators along a single axis of motion. This study uses a spatially-defined optogenetic control of a leading edge organizer to explore how neutrophil-like cells balance the “decisiveness” needed to polarize in a single direction with the flexibility needed to respond to new cues.