Abstract— The data of surface functionalization using bovine serum albumin of Ag2S semiconductor nanoparticles (quantum dots) obtained by the “green” synthesis method are presented. It was found that the luminescence maximum of Ag2S quantum dots shifts to the short-wavelength range (peaks within 270–350 nm) as a result of functionalization. The study of the morphology and size of the functionalized Ag2S quantum dots revealed a uniform surface without structural defects and a spherical morphology. The diameter of the functionalized quantum dots was approximately 20 nm, while the diameter of the nonfunctionalized ones was up to 10 nm. Conjugates of the functionalized Ag2S quantum dots with monoclonal antibodies were obtained (in particular, the TU-01 antibodies against α-tubulin, the main protein of microtubules). Spectrophotometric analysis showed that the luminescence spectrum of the obtained Ag2S–TU-01 bioconjugates had several maxima depending on the excitation. The maxima corresponded to the wavelength range of 410–470 nm. It was demonstrated that the obtained Ag2S–TU-01 conjugates can be used for visualization and study of microtubule organization, particularly in plant cells. Thus, for the first time, bioconjugates with monoclonal antibodies have been created using Ag2S quantum dots obtained via a green synthesis method, which significantly reduces the toxicity of such nanoparticles, allowing them to be considered effective luminescent probes in cell biological studies.
#Journal | Journal of the European Optical Society-Rapid Publications
"Investigating ultra-thin Ag and Au layers using #spectrophotometry and #AFM imaging"
✍️Riley Shurvinton et al.
#InstitutFresnel
➡️https://bit.ly/3Q1Bw9s
#EuropeanOptics #OpenAccess
JEOS-RP: Rapid progress in optics and photonics has broadened its application enormously into many branches, including information and communication technology, ...
#Woodcock has the brightest #feathers ever measured https://www.birdguides.com/articles/woodcock-has-the-brightest-feathers-ever-measured/
How #woodcocks produce the most brilliant white #plumage patches among the #birds https://www.biorxiv.org/content/10.1101/2022.12.09.519795v2
The reverse surface of the white #EurasianWoodcock fail feather tips reflected around 55% of light, as measured using #spectrophotometry. This is around five times brighter than #SnowyOwl and 10 times brighter than the feathers of #ArcticRedpoll, both known for their startling white plumage.
Exporting Data from Old Gear Through LCD Sniffing
[Jure Spiler] was at a flea market and got himself a spectrophotometer -- a device that measures absorbance and transmittance of light at different wavelengths. This particular model seems to be about 25 years old, and it's controlled by a built-in keyboard and uses a graphical LCD to display collected data. That might have been acceptable when it was made, but it wasn't enough for [Jure]. Since he wanted to plot the spectrophotometry data and be able to save it into a CSV file, hacking ensued.
He decided to tap into the the display communication lines. This 128×64 graphical display, PC-1206B, uses a 8-byte interface, so with a 16-channel logic analyzer, he could see the data being sent to the display. He even wrote decoder software - taking CSV files from the logic analyzer and using primitive optical recognition on the decoded pixels to determine the digits being shown, and drawing a nice wavelength to absorbance graph. From there, he set out to make a standalone device sniffing the data bus and creating a stream of data he could send to a computer for storage and processing.
[Jure] stumbled into a roadblock, however, when he tried to use an Arduino for this task. Even using a sped-up GPIO library (as opposed to notoriously inefficient digitalRead), he couldn't get a readout frequency higher than 80 KHz - with the required IO readout rate deemed as 1 MHz, something else would be called for. We do wonder if something like RP2040 with its PIO machinery would be better for making such captures.
At that point, however, he found out that there's undocumented serial output on one of the pins of the spectrophotometer's expansion port, and is currently investigating that, having shelved the LCD sniffing direction. Nevertheless, this serves as yet another example for us, for those times when an LCD connection is all that we can make use of.
We've seen hackers sniff LCD interfaces to get data from reflow ovens, take screenshots from Game Boys and even equip them with HDMI and VGA ports afterwards. With a skill like this, you can even give a new life to a vintage calculator with a decayed display! Got an LCD-equipped device but unsure about which specific controller it uses? We've talked about that!
#hardware #reverseengineering #graphiclcd #lcdcontroller #lcdmod #logicanalyzer #screenshots #screenshot #sniffing #spectrophotometer #spectrophotometry
Lab skills: 1/2-night beginners #classes in #NYC
Heya! I'm helping out with a community lab called Biotech without Borders. Part of this help will be teaching adult #science classes there to further the mission to "enable the #hands-on #practice of #biotechnology by communities, groups and countries hitherto underrepresented in the #biotech landscape"
I'm gonna start with two 1-night classes
On gel #electrophoresis:
https://www.eventbrite.com/e/lab-skills-night-how-to-cut-and-measure-dna-tickets-55879592365?aff=ebdssbdestsearch
On #spectrophotometry:
https://www.eventbrite.com/e/lab-skills-night-learn-spectrophotometry-tickets-55879673608?aff=ebdssbdestsearch
Prepare & Analyze a DNA molecular weight ladder How does a scientist know how large a fragment of DNA is? A routine answer is: by comparing it to other fragments of known sizes using a technique called gel electrophoresis. In this workshop, you will prepare your own DNA “ruler” by cutting a circular piece of DNA with enzymes called restriction endonucleases that act as tiny molecular scissors. The discovery of these enzymes in the 1970s was key to establishing the field of genetic engineering. Visualizing the resulting fragments of DNA on a gel is a technique used every day in labs all around the world. In this workshop you will get experience using restriction enzymes, pipettes, gel electrophoresis equipment, heat block and transilluminator to visualize your work. About Lab Skills Workshops: In this ongoing series of 1-2 day courses at Biotech without Borders, we’re inviting people of all skill levels together to complete a small teaching exercise in order to learn about laboratory work. We will cover the proper use of equipment and the real world application of the techniques being practiced. Instructor Danny Chan received his MSc in microbiology during the course of his PhD candidacy at the University of Chicago studying the interaction of methicillin resistant staphylococcus aureus (MRSA) with a model system of organotypic human keratinocytes in an infectious disease focused department. He has been a research technician for many years in multiple fields including cellulostic ethanol production, protein crystallography, prefrontal cortex development and heat shock proteins. The common set of skills underlying his practice are molecular biology and scientific inquiry which he aspires to apply to society in order to foster new institutions of research and learning. Currently, he makes his living fact checking pharmaceutical ads in an agency while trying to defend his time to pursue independent research centered around protocol development for the DIY science community. You can follow his activities online at danwchan.ca or find him in person around NYC.
German Virtual Observatory
Cytology and Genetics
edp sciences
Lukas VFN 🇪🇺
hackaday unofficial
Danny (he/they)