Neues in unserer #Methoden -Rubrik: Forschende an der University of Minnesota haben eine „One-Pot“-Methode entwickelt, die es ermöglicht #Klonierung und #Proteinexpression in einem Reaktionsgefäß durchzuführen!
Klingt vielversprechend? Finden wir auch! Mehr zu lesen im Artikel von Andrea Pitzschke:
👉 laborjournal.de/editorials/3368.php

#Biotechnologie #Laborjournal

Absolutely love this #preprint! The authors do cell-free #proteinexpression in the mRNA-display style to attach the encoding cDNA to the expressed protein. Then, they subject to proteolysis to infer information about protein stability.

#DNA #sequencing ties it together, and you have a 1.8-million variant library with quality stability data for ~850k of those.

Give it a look: https://www.biorxiv.org/content/10.1101/2022.12.06.519132v2.full.pdf

1L scale eukaryotic cell-free expression 👀

https://www.biorxiv.org/content/10.1101/2022.11.10.515920v1.full.pdf

The authors take a commercial system and just make a ton of tobacco cell lysate to show it scales linearly. Seems good.

They make several therapeutic proteins and even a VLP. Yields aren't going too have us tearing out bioreactors yet but the field is moving!

#bioprocess #proteinexpression #manufacturing #syntheticbiology

Referenced link: https://phys.org/news/2022-11-micrornas-boostrather-dampenprotein.html
Discuss on https://discu.eu/q/https://phys.org/news/2022-11-micrornas-boostrather-dampenprotein.html

Originally posted by Phys.org / @[email protected]: https://twitter.com/physorg_com/status/1590360075989680129#m

Surprisingly, these microRNAs boost—rather than dampen—#proteinexpression @ChemicalBiology https://pubs.acs.org/doi/10.1021/acscentsci.2c00748 https://phys.org/news/2022-11-micrornas-boostrather-dampenprotein.html