Prof Paula SalgadoMar 6
This week I went back to being student - and it was great! Fantastic to attend #cryoEM course at eBIC, co-organised with @[email protected]! I learned a lot and also realised my #proteincrystallography expertise are useful here too ;) can’t wait to apply what I learned!
StructBiolCommunicationsDec 17
The rigid single-domain structure and reproducible crystallization behavior of PF1765 from P. furiosus indicate potential use as a fusion domain to aid the crystallization of membrane proteins or complexes #PF1765 #UPF0235 #ProteinCrystallography https://doi.org/10.1107/S2053230X25009318
JanGebauerNov 28, 2024
Christmas is getting closer, and even our crystals are getting into the festive spirit! 😉 #ProteinCrystallography
David Aragão 🔶Sep 6, 2024

Practical on making videos and figures with mol* (https://molstar.org/)by Genevieve Evans @PDBeurope at the last day of the #CCP4 @britcryst #workshop run at @JohnInnesCentre

#crystallography #ccp4bca #molstar #proteincrystallography #norwich

Mol*

David Aragão 🔶Sep 5, 2024

Student and speaker geographic distribution at the #CCP4 BCA #crystallography #workshop running at the @JohnInnesCentre in #Norwich UK.

#proteincrystallography #structuralbiology #teaching #school #scattering

David Aragão 🔶Sep 5, 2024

Light talk 😊 to start the morning on experimental phasing by Airlie McCoy on the #CCP4 BCA #crystallography workshop running at the @JohnInnesCentre in #Norwich UK.

@britcryst #proteincrystallography #sfructuralbiology #phaseproblem #anomalous #scattering

Prof Paula SalgadoMar 20, 2024

I was flash cooling* crystals w/ AmSO4 today.
It was so cool to:
1. Do something I love again
2. Know I still got it! 😆

Can’t wait to see if cryo worked and they diffract! #proteincrystallography #PIinthelab

*not frozen. Speak to Elspeth Garman if you don’t know the difference

JanGebauerJun 14, 2023
Our protein crystallography course 2023 is in full swing. It's a nice group! However, I still get amazed that students can surprise me after 10y+ teaching. Never saw an diffraction image as shown. Prob the detector moved during exposure?🤔(that should definitely NOT happen 😬) #ProteinCrystallography #StructualBiology
Show threadStructBiolCommunicationsMar 30, 2023
One more Methods Communication: a computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals is described and its performance is analysed #ProteinCrystallography #Cryocrystallography #DataCollection https://doi.org/10.1107/S2053230X2001420X
Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals

Cryocrystallography, which is commonly used in macromolecular crystallography, may sometimes reduce the quality of diffraction data and the visibility of crystals owing to frost adhesion. A device has been developed to remove frost by drizzling liquid nitrogen over the crystals, which enabled noise reduction of diffraction images and the centering of crystals with low visibility owing to frost adhesion.

Acta Crystallographica Section F
Show threadStructBiolCommunicationsMar 24, 2023
This Methods Communication describes an in situ diffraction measurement system that can collect small-wedge data from crystals in a drop on a plate at room temperature #ProteinCrystallography #InSituXRayDiffraction #RoomTemperatureDataCollection https://doi.org/10.1107/S2053230X22005283
In situ crystal data-collection and ligand-screening system at SPring-8

An in situ X-ray diffraction measurement system using a crystallization plate has been constructed at the SPring-8 protein crystallography beamline. Utilizing small-wedge measurements and incorporating a liquid dispenser to prepare protein–ligand complex crystals, this system will make ligand screening possible.

Acta Crystallographica Section F