Precise deletion, replacement and inversion of large DNA fragments in plants using dual prime editing | Nature Plants

Precise manipulation of genome structural variations holds great potential for plant trait improvement and biological research. Here we present a genome-editing approach, dual prime editing (DualPE), that efficiently facilitates precise deletion, replacement and inversion of large DNA fragments in plants. In our experiments, DualPE enabled the production of specific genomic deletions ranging from ~500 bp to 2 Mb in wheat protoplasts and plants. DualPE was effective in directly replacing wheat genomic fragments of up to 258 kb with desired sequences in the absence of donor DNA. Additionally, DualPE allowed precise DNA inversions of up to 205.4 kb in wheat plants with efficiencies of up to 51.5%. DualPE also successfully edited large DNA fragments in the dicots Nicotiana benthamiana and tomato, with editing efficiencies of up to 72.7%. DualPE thus provides a precise and efficient approach for large DNA sequence and chromosomal engineering, expanding the availability of precision genome-editing tools for crop improvement. This study explores a precise and efficient chromosome editing technology that enables the scarless deletion, replacement and inversion of large DNA fragments, up to the megabase scale, in plants and holds considerable promise for crop improvement.

A Gene Editor to Correct a Common Cystic Fibrosis Mutation | Genetics And Genomics

Cystic fibrosis (CF) is a common genetic disorders that has been well studied. Researchers have identified mutations in a gene called CFTR that cause CF | Genetics And Genomics

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3rd Albrecht Müller ARRIGE Seminar – ARRIGE

Prime editing shows proof of concept for treating sickle cell disease

Genomic prime editing, a new DNA-changing technology, fixed sickle cell disease mutations in patient cells. Learn how it could lead to cure for genetic anemias.

Prime editing for precise and highly versatile genome manipulation - Nature Reviews Genetics

In this Review, Chen and Liu discuss the latest developments in prime editing systems, including improvements to their editing efficiency and capabilities, as well as diverse emerging applications in research and preclinical therapeutic studies.