4/n Loss of miR-33 in macrophages improves mitochondrial homeostasis (function and structure) at baseline and after bleomycin injury, ⬆️ autophagy and mitophagy
#CureIPF #miR33PFPaperhttps://insight.jci.org/articles/view/158100
JCI Insight -
microRNA-33 deficiency in macrophages enhances autophagy, improves mitochondrial homeostasis, and protects against lung fibrosis
3/n Knocking down miR-33 genetically in myeloid cells, or pharmacologically using a Peptide Nucleic Acid inhibitor designed by Dr. Bahal (PNA-33) protects mice against bleomycin-induced Pulmonary Fibrosis
#CureIPF #miR33PFPaperhttps://insight.jci.org/articles/view/158100
JCI Insight -
microRNA-33 deficiency in macrophages enhances autophagy, improves mitochondrial homeostasis, and protects against lung fibrosis
5/n Impressively, loss of Mir-33 in myeloid cells, has an effect on alveolar type II cells, their mitochondrial homeostasis improved and apoptosis ⬇️
#CureIPF #miR33PFPaperhttps://insight.jci.org/articles/view/158100
JCI Insight -
microRNA-33 deficiency in macrophages enhances autophagy, improves mitochondrial homeostasis, and protects against lung fibrosis
7/n So grateful to the team, led by Dr. Ahangari,
#KaminskiLab members, so many selfless collaborators including Drs Fernández-Hernando, Price, Dela Cruz, Prasse, Ivan Rosas, & Bahal (
https://bahal.lab.uconn.edu/ that designed PNA-33)
#CureIPF #miR33PFPaperhttps://insight.jci.org/articles/view/158100
Home | The Bahal Research Lab
Bahal Lab Dr. Bahal’s research lab integrates chemical biology of nucleic acid analogues and new polymeric delivery strategies to target genetic diseases and various kinds of cancer. His group’s major research interests include (1) selective targeting of microRNA and mRNA using antisense b ...
8/n last - Study funding was by @
[email protected] #DiscoveryAward to @
[email protected] & by @
[email protected] to me & others, also supported by institutional cores, colleagues, patient advocates, sample & tissue donors
Thank you all!!!
#CureIPF #miR33PFPaperhttps://insight.jci.org/articles/view/158100
JCI Insight -
microRNA-33 deficiency in macrophages enhances autophagy, improves mitochondrial homeostasis, and protects against lung fibrosis
6/n Pharmacologic inhibition of miR-33 using PNA-33 in precision-cut lung slices obtained from bleomycin-injured mice or humans with IPF, also indicated antifibrotic effects, including reversal of profibrotic macrophage signature
#CureIPF #miR33PFPaperhttps://insight.jci.org/articles/view/158100
JCI Insight -
microRNA-33 deficiency in macrophages enhances autophagy, improves mitochondrial homeostasis, and protects against lung fibrosis
1/n Our first
#CurePF paper for 2023 is OUT! A novel role in Pulmonary Fibrosis for miR-33, a key regulator of macrophage immuno-metabolic response & mitochondrial function, providing evidence that inhibition may be antifibrotic
#miR33PFPaperhttps://doi.org/10.1172/jci.insight.158100
JCI Insight -
microRNA-33 deficiency in macrophages enhances autophagy, improves mitochondrial homeostasis, and protects against lung fibrosis
2/n We first show that miR-33 is increased in BAL and lung cells obtained from IPF patients and that miR-33 target genes are decreased in IPF
#CureIPF #miR33PFPaperhttps://insight.jci.org/articles/view/158100
KaminskiMed 
