Mastodawn

#goose #goosewasnotvalued #matrix #keanu #reeves #nospoon

I just have been reminded about important thing

We, software developers and other IT specialists, often speak about dencentralization and censorship resistance, privacy or whatever else, that sounds important for those who understands how all this work. But for like 99.99% people it is something like a mumbo jumbo of some secret society or ritualistic jargon of some conspiracy theorists group. If it is necessary ever to get someone aboard of e. g. Fediverse or Matrix/Element, it would require to go completely different way. Software we […]

https://legends.house/i-just-have-been-reminded-about-important-thing/

I just have been reminded about important thing

House of Legends

Amᵃᵖanda 2h ago

Free your map

Open Street Map

#Matrix #OpenStreetMap #FreeYourMap

ebel aurora 2h ago

OMG! A #Matrix image / logo generator!
https://pixelframe.design/the-matrix-logo-font-generator/

Milo | Ende der Illusion 4h ago

Wir nennen die Matrix normal. Dabei steckt sie in allem: Regeln, Rollen, Angst, Kontrolle, Streit. Wir kommen hier hinein, und alles wirkt bereits fertig.
Wer anfängt zu fragen, kann nicht mehr aufhören.

#Matrix #EndeDerIllusion

Oscloud 14h ago

✅ Plánovaná údržba mxchat.cz je hotová. Server je zpět online, upgrade Debianu proběhl úspěšně. Děkujeme za trpělivost! #mxchat #OSCloud #Matrix

etke.cc 20h ago

Weekly Recap https://etke.cc/news/2026-06-25-weekly-recap/

#matrix

etke.cc | Weekly Recap

New message published on 2026-06-25 20:00 UTC

etke.cc

Garbage Data 🦝20h ago

I'm finally on #Matrix

@ garbagedata:pawb.social

BlablaLinux 1d ago

🔥 Alerte mises à jour du soir ! 🛠️

Vos services libres et auto-hébergés préférés font le plein de nouveautés :

💬 #Cinny & #Element – Salons #Matrix mis à jour.
🐘 #Mastodon – Prêt pour vos meilleurs pouets.
🔗 #Snowshare – Partage sécurisé de fichiers, liens et code au top.
📄 #StirlingPDF – Le couteau suisse du PDF est affûté.

Tout est en ligne et stable ! Bonne navigation à tous 🚀

Retrouvez mes services sur https://blablalinux.be/mes-services-publics/.

BlablaLinux Mes services PUBLICS BlablaLinux : état, descriptions et liens

Découvrez la liste complète des services PUBLICS que j’auto-héberge avec Blabla Linux (Nextcloud, Mastodon, Gitea…) et consultez leur état de santé en temps réel.

BlablaLinux

Rxiv mechanobio 1d ago

📰 "CRISPR interference functional genomics of coding and non-coding determinants of Bacillus subtilis biofilms"
https://www.biorxiv.org/content/10.64898/2026.06.23.734000v1?rss=1 #Dynamics #Matrix

CRISPR interference functional genomics of coding and non-coding determinants of Bacillus subtilis biofilms

The architecture of Bacillus subtilis biofilms is influenced by the coordinated regulation of cellular specialization, matrix assembly, and metabolism. B. subtilis can form different types of biofilm in diverse physical and chemical environments. Understanding the molecular mechanisms that drive biofilm heterogeneity and adaptation to different environmental niches is crucial for developing more effective strategies to control their formation. In this study, we developed a tightly dual-regulated CRISPR interference (CRISPRi) system and employed multi-scale imaging to investigate the functions of individual genes in two distinct biofilm models: the floating pellicle and the intricate, three-dimensionally structured macrocolony, which develop at the liquid-air and solid-air interfaces, respectively. Our findings validated the CRISPRi approach as a powerful method for studying biofilm development over extended periods and revealed that numerous small non-coding RNAs are involved in regulating biofilm growth dynamics and architecture. The CRISPRi approach was also applied to a pool of 507 genes and transcription units, including protein-coding genes and non-coding RNAs, to screen for cell fitness in these two biofilm models. We discovered that, while both biofilm forms rely on fundamental processes such as cell wall synthesis and nucleotide metabolism, they exhibit different genetic dependencies with regard to matrix composition, motility, and signaling. Exopolysaccharide production, motility, and chemotaxis are crucial for pellicle formation. In contrast, macrocolony development is influenced by γ-polyglutamate synthesis and nutrient acquisition functions. Genes of unknown function were also identified to play a differentially important role in the two biofilm forms. Additionally, the CRISPRi screens revealed further non-coding RNAs regulating biofilm architecture and growth dynamics, adding to the existing layers of post-transcriptional control. Collectively, these results demonstrate that biofilm formation at different physical interfaces is governed by a combination of shared and unique genetic pathways tailored to the specific biofilm environment, thereby opening research avenues into the molecular mechanisms specific to the solid-air and liquid-air interfaces. ### Competing Interest Statement The authors have declared no competing interest.

bioRxiv