Microscopy lectures in video:
https://www.ibiology.org/online-biology-courses/microscopy-series/

About cameras, instruments, point-spread functions, dyes, the photon budget, and a lot more, from Lichtmann, Vale, Smith, Hell, Stuurman, Tsien, Lippincott-Schwartz and a lot more.

#microscopy #LightMicroscopy

Microscopy Course on iBiology

This popular, free online microscopy course begins with basics of optics, proceeds through transmitted light microscopy, and covers many microscopy methods.

iBiology

Florian Jug's lab @florianjug latest:

"MicroSplit: semantic unmixing of fluorescent microscopy data", Ashesh et al. 2026
https://www.nature.com/articles/s41592-026-03082-1

"MicroSplit separates up to four superimposed noisy structures into distinct, denoised image channels, enabling faster and more photon-efficient imaging."

"Built on Variational Splitting Encoder-Decoder networks, models a posterior distribution over solutions, allowing uncertainty-aware predictions and the estimation of spatially resolved prediction errors from posterior variability."

#BioimageInformatics #LightMicroscopy #microscopy

$${\bf{Micro}}{{\mathbb{S}}}{\bf{plit}}$$ Micro S plit : semantic unmixing of fluorescent microscopy data - Nature Methods

$${\rm{Micro}}{\mathbb{S}}{\rm{plit}}$$ M i c r o S p l i t is a computational unmixing method for multiplexed fluorescence imaging of up to four structures per imaging channel.

Nature

Interactive Paraxial Kohler Illumination Simulation, by David Nguyen, for teaching purposes:
https://kohler-simulation.microscopybench.com/

Explained here:
https://forum.image.sc/t/interactive-paraxial-kohler-illumination-simulation/119906

#microscopy #LightMicroscopy

Köhler Illumination

Superb technique:

"Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p", Majnik et al. 2025
https://elifesciences.org/articles/107540

#neuroscience #mouse #TwoPhotonMicroscopy #LightMicroscopy #Track2p

Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p

A novel algorithm allowed for the continuous tracking of neurons throughout early postnatal development, revealing a transition in neural firing statistics coinciding with the onset of behaviour-dependent activity.

eLife

Position open: Head of the Center for Microscopy and Image Analysis (Core Facility) at the Center for Microscopy and Image Analysis (ZMB) in the University of Zurich, Switzerland.

https://www.nature.com/naturecareers/job/12847076/head-of-the-center-for-microscopy-and-image-analysis-core-facility-/

#microscopy #PhDJobs #academia #ElectronMicroscopy #LightMicroscopy #ImageAnalysis

Head of the Center for Microscopy and Image Analysis (Core Facility) - Canton of Zürich (CH) job with University of Zurich (UZH) | 12847076

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Nature Careers
Opal mineraloid viewed under a smartphone-based do-it-yourself microscope 🔬

Image width is 3 millimeters 📏 and obtained by white light reflection.

#science #mineraloid #opal #lightmicroscope #lightmicroscopy #microscope #microscopy #microscopyart #lens #doityourself #doityourselfexperiment #buildityourself
Agate mineral viewed under a smartphone-based do-it-yourself microscope 🔬

Image width is 3 millimetres 📏 and obtained by white light reflection.

#science #mineral #mineralogy #agate #lightmicroscope #lightmicroscopy #microscope #microscopy #microscopyart #lens #doityourself #doityourselfexperiment #buildityourself
Opal mineraloid viewed under a smartphone-based do-it-yourself microscope 🔬

Image width is 3 millimeters 📏 and obtained by white light reflection.

#science #mineraloid #opal #lightmicroscope #lightmicroscopy #microscope #microscopy #microscopyart #lens #doityourself #doityourselfexperiment #buildityourself
Agate mineral viewed under a smartphone-based do-it-yourself microscope 🔬

Image width is 3 millimetres 📏 and obtained by white light reflection.

#science #mineral #mineralogy #agate #lightmicroscope #lightmicroscopy #microscope #microscopy #microscopyart #lens #doityourself #doityourselfexperiment #buildityourself #wavy #creamy