Complete Mitochondrial Genome of the Ossuaria sichuanensis (Hemiptera: Cicadellidae: Typhlocybinae) from China and Its Phylogenetic Implications - Cytology and Genetics

Abstract Leafhoppers, a broad group of Hemiptera insects, are economic pests that wreak havoc on agriculture and forestry. The genus Ossuaria was established by Dworakowska in 1979, Zhang and Yang discovered Ossuaria sichuanensis (Zhang and Yang, 2011) from China in 2011, but there is no molecular biological evidence to record the genus. To report complete mitochondrial genome of O. sichuanensis, DNA was extracted from the muscle tissue of adult insect and sequenced. The original data were assembled and annotated, a complete mitochondrial genome was mapped, and phylogenetic analysis was performed using maximum likelihood and Bayesian inference. The circular mitogenome of O. sichuanensis is 15.558 bp in length and including 37 typical genes (13 PCGs, 22 tRNA genes, and 2 rRNA genes) and a putative control region. The overall base composition is 42.89% A, 36.92% T, 11.02% C, and 9.17% G, and the A + T content is 79.81%. Phylogenetic analyses confirmed the O. sichuanensis is the sisters of other members of the tribe Erythroneurini. The mitochondrial genome structure agrees with earlier research. The genus Ossuaria has been characterized at the molecular level for the first time, and additional research is required to determine how the tribe Zyginellini and the tribe Typhlocybini are related.