Thrilled to see our work featured on the cover of Molecular Cell.
The image: an electron micrograph of an i-loop, the DNA structure we propose as a source of eccDNA formation. Almost like catching circular DNA formation in the act.
#MolecularCell #eccDNA #ecDNA #CancerResearch #GenomeInstability #DNARepair @MolecularCell
Huge congratulations to @EZ_eta for leading the experimental work, and a big thank you to our collaborators at IIT for their Nanopore expertise, as well as to Michele Giannattasio head of the EM on Single Molecules Facility for his contribution to the structural analysis.
#eccDNA #ecDNA #CancerResearch #GenomeInstability #DNARepair #ReplicationStress
How do cancer-associated extrachromosomal circular DNAs arise?
In our new manuscript, we show that single-strand DNA damage in repetitive sequences can trigger i-loop formation and eccDNA generation. What started at telomeres may reflect a much broader principle across the genome and help explain how ecDNA may emerge in cancer.
https://authors.elsevier.com/a/1n0wG3vVUPZO38
#eccDNA #ecDNA #CancerResearch #GenomeInstability #DNARepair #ReplicationStress
Our recent Perspective on extrachromosomal circular DNA is the focus of December's GBE Highlight.
Highlight: Running Circles Around Genome Architecture
🔗 https:/doi.org/10.1093/gbe/evaf232
This is our first Highlight produced under the SMBE Fellows Program, written by Shady A. Kuster.
From 2022:
Extrachromosomal circular DNA: A neglected nucleic acid molecule in plants.
#ExtrachromosomalCircularDNA #eccDNA #Transposons #PlantScience
https://www.sciencedirect.com/science/article/pii/S1369526622000929
A comprehensive atlas of full-length Arabidopsis eccDNA populations identifies their genomic origins and epigenetic regulation.
#ExtrachromosomalCircularDNA #eccDNA #Transposons #Arabidopsis #MethylationMutants #LongReadSequencing
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3003275
Previous studies of extrachromosomal circular DNA (eccDNA) in plants are based on short-read sequencing and cannot efficiently identify their full-length, generated from transposable elements. This study uses long-read sequencing to obtain a comprehensive map of full-length eccDNAs from Arabidopsis and identifies their genomic origins and epigenetic regulation.
#DNA element with a murky past is borrowing cell's #repair machinery.
#retrotransposon #parasite #alt_EJ #eccDNA
https://phys.org/news/2023-07-dna-element-murky-cell-machinery.html
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