katch wreck"Here we introduce double #emulsion droplet sensors that enable local measurements of #osmotic pressure intra- & extra-cellularly within 3D multicellular systems, including living tissues. After generating & calibrating the sensors, we measure the osmotic pressure in #blastomeres of early #zebrafish embryos as well as in the interstitial fluid between the cells of the #blastula by monitoring the size of droplets previously inserted in the embryo"
In situ quantification of osmotic pressure within living embryonic tissues - Nature Communications
Osmotic pressure is thought to play a key role in many cellular and developmental processes, but remains challenging to measure it in cells and tissues. Here, the authors present a sensor based on double emulsion droplets that allows quantification of osmotic pressure in situ and in vivo.