David SchnettlerMay 6, 2024

If you've ever done a bit of #ProteinEngineering or #DirectedEvolution you know that funny things can happen 🤯 – and sometimes it's worth following up!

I'm excited to share that our paper is finally out in Nature Chemistry! 🎉

Read it here:
https://www.nature.com/articles/s41557-024-01490-4

Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins - Nature Chemistry

Evolution separates complex modern enzymes from their hypothetical simpler early ancestors, which raises the question of how unevolved sequences can develop new functions. Here a library of non-natural protein sequences was subjected to ultrahigh-throughput screens in microfluidic droplets, leading to the isolation of a phosphodiesterase enzyme capable of hydrolysing the biological second messenger, cyclic AMP.

Nature
ChemistryViewsApr 5, 2024

An Enantioselective Biocatalytic C–H Fluorination
Directed evolution used to create a fluorinating enzyme

https://www.chemistryviews.org/an-enantioselective-biocatalytic-c-h-fluorination/

#chemistry #chemistryviews #chemiverse #research #directedevolution #biocatalysis #fluorination

An Enantioselective Biocatalytic C–H Fluorination - ChemistryViews

Directed evolution used to create a fluorinating enzyme

ChemistryViews
ChemistryViewsDec 13, 2023

Directed Evolution Used to Create Enzymes That Break Down Plastics:
Polyethylene terephthalate (PET)-depolymerizing enzyme developed using new high-throughput screening

https://www.chemistryviews.org/directed-evolution-used-to-create-enzymes-that-break-down-plastics/

#chemistry #chemistryviews #chemviews #chemiverse #research #directedevolution #breakdownplastic #PlasticPollution

Directed Evolution Used to Create Enzymes That Break Down Plastics - ChemistryViews

Polyethylene terephthalate (PET)-depolymerizing enzyme developed using new high-throughput screening

ChemistryViews
Victoria Stuart 🇨🇦 🏳️‍⚧️Sep 8, 2023

Emergent biosynthetic pathway to essential amino acids by metabolic metathesis
https://www.biorxiv.org/content/10.1101/2023.09.06.556532v1?rss=1

* alkene metathesis unknown in extant biochemistry
* alkenic amino acid vinylglycine can be generated in biocompatible reaction
* vinylglycine produced in situ then used as precursor of isoleucine & methionine
* allows growth of Escherichia coli strains normally requiring these essential amino acids

#biochemistry #MolecularBiology #SyntheticBiology #DirectedEvolution #auxotrophy #prototrophy

societyoutcastsFeb 6, 2023
DEVELOPING: Pfizer Disables Comments After Video Showing #DirectedEvolution
https://youtube.com/shorts/YKiihra6soE?feature=share
DEVELOPING: Pfizer Disables Comments After Video Showing #DirectedEvolution

YouTube
tjitjakJan 30, 2023

I think it is time to stop using #pfizer #vaccines anymore. Or any #US #vaccines for that matter

Just watch the video ...
---
RT @Project_Veritas
BREAKING: @pfizer Exploring "Mutating" COVID-19 Virus For New Vaccines

"Don't tell anyone this...There is a risk...have to be very controlled to make sure this virus you mutate doesn't create something...the way that the virus started in Wuhan, to be honest."

#DirectedEvolution
https://twitter.com/Project_Veritas/status/1618405890612420609

Project Veritas on Twitter

“BREAKING: @Pfizer Exploring "Mutating" COVID-19 Virus For New Vaccines "Don't tell anyone this...There is a risk...have to be very controlled to make sure this virus you mutate doesn't create something...the way that the virus started in Wuhan, to be honest." #DirectedEvolution”

Twitter
Surabaya JohnnyJan 28, 2023

https://youtu.be/sFStX5JH_8Y

#directedevolution

[complaints about the content of my troeets, please send to the zoo direction]

GUZ - Genforscher

YouTube
KriesLabJan 26, 2023
#DirectedEvolution is a powerful method but has not been easy to do with gigantic #nonribosomal biocatalysts that make important #antibiotics. We have developed a #microfluidics screen that uses liposomes as sensors for membrane active peptides. Bacteria are grown in tiny droplets. If they make gramicidin S, the sensor liposomes burst, liberate calcein, and generate fluorescence. This was tested for screening mutants and may become a useful tool for directed evolution. https://www.biorxiv.org/content/10.1101/2023.01.13.523969v1
phicyclistJan 26, 2023

Knowingly.
---
RT @Project_Veritas
BREAKING: @pfizer Exploring "Mutating" COVID-19 Virus For New Vaccines

"Don't tell anyone this...There is a risk...have to be very controlled to make sure this virus you mutate doesn't create something...the way that the virus started in Wuhan, to be honest."

#DirectedEvolution
https://twitter.com/Project_Veritas/status/1618405890612420609

Project Veritas on Twitter

“BREAKING: @Pfizer Exploring "Mutating" COVID-19 Virus For New Vaccines "Don't tell anyone this...There is a risk...have to be very controlled to make sure this virus you mutate doesn't create something...the way that the virus started in Wuhan, to be honest." #DirectedEvolution”

Twitter
Sir R DanielsJan 26, 2023

BREAKING: Pfizer Exploring "Mutating" COVID-19 Virus For New Vaccines

"Don't tell anyone this...There is a risk...have to be very controlled to make sure this virus you mutate doesn't create something...the way that the virus started in Wuhan, to be honest."

#DirectedEvolution

https://www.youtube.com/watch?v=ywlpArNWKxM

Pfizer Exposed For Exploring "Mutating" COVID-19 Virus For New Vaccines Via 'Directed Evolution'

YouTube