Optimizing #cytochrome #P450 network for high-level production of #quillaic_acid.
https://phys.org/news/2023-10-optimizing-cytochrome-p450-network-high-level.html
Researchers from the Shenzhen Institute of Advanced Technology of the Chinese Academy of Sciences have developed a combinatorial optimization approach to construct and spatially control a cytochrome P450-cytochrome P450 reductase (CYP-CPR) network in an engineered Saccharomyces cerevisiae strain to boost quillaic acid production.
" 5-Aminolevulinic acid (5-ALA) is a crucial precursor of heme, which is essential for CII, complex III, complex IV (CIV) and #cytochrome c activities. Here, we show that feeding a combination of 5-ALA hydrochloride and sodium ferrous citrate (5-ALA-HCl + SFC) increases #ATP production and suppresses defective phenotypes in #Drosophila with Complex I (CI) deficiency."
https://academic.oup.com/hmg/advance-article/doi/10.1093/hmg/ddad092/7207871
"Here, using cryoelectron microscopy, we describe the atomic structures of two extracellular #cytochrome #nanowires (ECNs) from two major orders of hyperthermophilic #archaea present in deep-sea hydrothermal vents and terrestrial hot springs...The detection of ECNs in archaea suggests that filaments containing closely stacked hemes may be a common and widespread mechanism for long-range #electron transfer in both #prokaryotic domains of life."
#Extracellular #cytochrome #nanowires appear to be ubiquitous in #microbes.
#bacteria #archea #Geobacter #bioinformatics #electron_transport #Pyrobaculum
https://phys.org/news/2023-07-extracellular-cytochrome-nanowires-ubiquitous-microbes.html
A Geobacter bacteria isolated from a contaminated ditch in Oklahoma has an unusual appendage—a long extracellular nanowire that can conduct electricity. The electron transport chain in this nanowire carries electrons from the bacteria to an insoluble external electron acceptor to help the microbe make energy. Such long-range electron transfers at a micron-scale are thought to have played an important role in microbial metabolism on the early Earth.
Cytochrome C is a complex mitochondrial protein involved in energy production and cell destruction. But also likely a lot more we haven't found yet.
After finding and accidentally losing a new conformation of it in 2009, Uruguay scientists along with Vanderbilt, they have re-isolated it using a specially built monoclonal antibody.
#Science #SciComm #Genetics #Biochemistry #Cytochrome #Mitochondria #Proteins #Uruguay #Vanderbilt
An Ultra-Stable Protein Nanowire Made by Bacteria https://microbiologycommunity.nature.com/posts/an-ultra-stable-protein-nanowire-made-by-bacteria-provides-clues-to-combating-climate-change
Structure of #Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity https://www.nature.com/articles/s41564-022-01315-5
#Bacteria deep in the #soil need #nanowires made up of #cytochrome OmcZ to export electrons to extracellular acceptors in order to survive without oxygen. New research explains how bacteria make nanowires on demand and why only OmcZ nanowires show extremely high electron #conductivity.
Bacteria deep in the soil need "nanowires" made up of cytochrome OmcZ to export electrons to extracellular acceptors in order to survive without oxygen. Our cryo-EM OmcZ architecture explains how bacteria make nanowires on demand and why only OmcZ nanowires show extremely high electron conductivity.
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