🧠 New paper by Carneiro-Nascimento et al: awake #invivo #2p #CalciumImaging reveals diverse #CalciumDynamics in #meningeal #macrophages during cortical spreading depolarization (CSD), an aberrant #brain #hyperexcitability event linked to #migraine, #stroke, and #traumatic brain injury.

📝 https://doi.org/10.7554/eLife.109888

#Neuroimmunology #Neuroscience #imaging @eLife https://fediscience.org/@eLife/116605475951455191

🧠 New paper by Phillips et al: #Neuroplex combines #miniscope #CalciumImaging with multiplexed #SpectralImaging through the same #GRINlens, allowing up to 9 projection-defined #NeuronalPopulations to be identified in freely #behaving mice. Instead of measuring activity alone, the approach links #FunctionalDynamics directly to circuit identity within the same animal and behavioral session.

📝 https://doi.org/10.7554/eLife.110277

#Neuroscience #CompNeuro #NeuralCircuits #Imaging @eLife https://fediscience.org/@eLife/116589668668928682

Recent findings show that hippocampal neurons shift their activity backward in time as learning occurs, enabling anticipation of rewards before they happen. Using calcium imaging in mice performing a delayed nonmatching-to-location task, the study tracked the same neurons over weeks and observed a backpropagation of tuning from reward delivery to earlier moments, including the moment of correct touchscreen choice. The results portray the hippocampus as a dynamic predictive map that reorganizes with experience to guide future behavior.

This work is of interest to psychology because it provides concrete neural evidence for predictive coding and memory-based anticipation, illustrating how learning reshapes mental representations to forecast future events. It links memory, prediction, and action in a measurable neural framework.

Article Title: Hippocampal neurons shift their activity backward in time to anticipate rewards

Link to PsyPost Article: ift dot tt/fVBpnxF

Copy and paste broken link above into your browser and replace "dot" with "." for link to work. We have to do it this way to avoid displaying copyrighted images.

#Hippocampus #PredictiveCoding #NeuralPlasticity #CalciumImaging #RewardLearning

🧠 New preprint by Kim et al. (2025) from David Anderson’s lab: A line #attractor maintains aggressiveness during feeding in “hangry” mice 🍔🐁. Using in vivo #CalciumImaging and #rSLDS modeling, they show how moderate fasting stabilizes an aggression-related attractor in #VMHvl, while prolonged fasting collapses it, linking hunger, motivation, and aggression through #PopulationDynamics:

🌍 https://doi.org/10.1101/2025.10.16.682711

#Neuroscience #CompNeuro #Behavior #AttractorDynamics #Hypothalamus #2p #imaging

If you want to study #HippocampalReplay... Use ephys, not #CalciumImaging!!

(Calcium imaging doesn't detect single spikes well, but replay mostly involves single spikes)
#Neuroscience #SpatialCognition #Hippocampus

New #TeachingMaterial available: Functional Imaging Data Analysis – From Calcium Imaging to Network Dynamics. This course covers the entire workflow from raw #imaging data to functional insights, including #SpikeInference & #PopulationAnalysis. Designed for students and for self-guided learning, with a focus on open content and reproducibility. Feel free to use and share it 🤗

🌍 https://www.fabriziomusacchio.com/blog/2025-07-13-function_image_analysis/

#Python #DataScience #MachineLearning #Neuroscience #OpenSource #calciumimaging #CompNeuro

📢 Our new study is now published in Communications Biology (Nature Portfolio):
We demonstrate deep in vivo #ThreePhoton imaging 🔬 of neurons 🧠 and glia in the medial prefrontal cortex with subcellular resolution!

👉 https://www.nature.com/articles/s42003-025-08079-8

#Neuroscience #Microscopy #CalciumImaging #Microglia #DZNE @dzne

📢Hot off the press: "Neuronal correlates of sleep in honey bees"

#CalciumImaging🔬 in sleeping #bees🐝: Antennal lobe neurons synchronise stronger during #sleep, likely due to reduced GABAergic coupling. #SNN💻 simulations show reduced #odour processing, similar to human sleep😴.

📰in Neural Networks: https://doi.org/10.1016/j.neunet.2025.107575

🍾Thanks to all collaborators: Sebastian Moguilner, Ettore Tiraboschi, Giacomo Fantoni, Heather Strelevitz, Hamid Soleimani, Luca Del Torre, @urihasson

📍#CIMeC #UniTrento

Quite the big deal:

"Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging", by Howe et al. 2025 (Amanda Foust lab).
https://www.biorxiv.org/content/10.1101/2025.03.17.643718v1.full

Transfers one-photon light field images of Ca2+ sensors monitoring neuronal activity, which suffer from scattering in the mouse brain, to two-photon volumes that don't, using machine learning.

Image volumes acquired at 100 Hz demonstrate 10Hz spike rates.

#neuroscience #mouse #CalciumImaging