Elisa Fadda-πΊBen Schumann presents recent results on and obtained with the glyco #bioorthogonal tools developed in his lab. Bump-and-hole engineering FTW πππππ₯π₯ #glycotime #chemiverse #chemistry
Jeremy Baskinπ¨New manuscript: interorganelle lipid transport is rapid, & it is hard to quantify discrete steps in this network, mediated by lipid transfer proteins working at membrane contact sites between different organelles. We (Dongjun Liang & Lin Luan) harness chemoenzymatic tagging & super-fast #bioorthogonal chemistry to make fluorescent lipids that report on PM-to-ER phospholipid flux mediated by a family of lipid transfer proteins at these contact sites! https://doi.org/10.1101/2023.02.01.526230 #lipidtime #chembio
Ben SchumannThrilled to share this story in JACS Au! https://pubs.acs.org/doi/10.1021/jacsau.2c00419
Takeaways:
1) analogs of PC lipids w/different choline structures adopt different organelle localizations & are useful for tracing interorganelle lipid transport
2) OCT1, a liver transporter typically used to study PK/drug efflux, can also facilitate uptake of small cationic #ChemicalBiology probes, including #bioorthogonal choline analogs. Available soon @Addgene https://addgene.org/188995/
Congrats, Dylan!
#lipidtime #OpenAccess
Brett GarabedianHi #mastodon
Suppose It's high time for my #introduction π I'm a 4th year grad student working with Jim Paulson @ Scripps Research where I develop therapies targeting the #SialicAcid immune axis to combat #cancer & viral infection.
Besides #glycans & #glycotime (obvi.) recent interests include #ProteinEngineering, #Phage Display, #DELs, #TPD, #Bioorthogonal Chemistry and #ChemBio at large.
Intrigued by the #startup life & low-key shopping around for #postdoc ops
#mastodonmigration π
Verena StraubThis 2021 article was shared by RSC chemical biology for #FluorescenceFriday:
https://pubs.rsc.org/en/content/articlelanding/2021/cb/d1cb00002k
Tbh I was not aware of click-based amplification techniques and this paper hasn't been cited yet or gained much attention otherwise.
The research this was based on has been applied only to nucleic acid imaging:
https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.201700209
So the question is has anyone tried this? Does it work and is it useful?
Click-based amplification: designed to facilitate various target labelling modes with ultralow background amplification
We here describe a fluorescent signal amplification method termed βClick-based amplificationβ that can be well integrated with various click-labelling modes, including chemical labelling, genetic incorporation and covalent inhibitor probe mediated target labelling. Picolyl azide (pAz) was used as a functiona
Carolyn BertozziACS Chem Biol put together a special issue on #bioorthogonal and #clickchemistry, great selection of articles that touch upon every biomolecule type with far-ranging applications in biology and medicine. Well done!
https://pubs.acs.org/page/acbcct/vi/click-bioorthogonal-chemistry
Gerling-Driessen Lab#introduction to our work. Trained as an organic chemist, I found my passion for #glycotime working with @bertozzi at stanford university on understanding #raredisease #NGLY1 deficiency during my postdoc. With my team, currently located at Heinrich Heine University in Duessendorf, I use #bioorthogonal chemistry to study aberrant #glycosylation in congenital disorders of glycosylation and some types of cancer. We develop multi-modal chemical probes for #imaging and #glycoproteimics
Hi everyone! I'm @basktastic on Twitter but am trying out my real name here. My #chembio #cellbio #lipidtime lab at Cornell develops tools for imaging and editing the lipidome, drawing on #bioorthogonal chemistry, #clickchemistry, #chemoproteomics, #optogenetics, #directedevolution, and more. https://baskin.wicmb.cornell.edu
Hi everyone, I am new here and learning the platform. By way of background, I am a chemical biologist and glycoscientist on the Stanford Chemistry faculty. Known for creating a field called #bioorthogonal chemistry (for which I shared the 2022 NP) and launching biotech companies with new therapeutic and diagnostic platforms, mostly rooted in glycobiology. Passionate about interdisciplinary science, diversity equity and inclusion in STEM, peanut butter, gyms and 70s funk.