Ron Beavis ❌Jan 24, 2024
A GPMDB ubiquitination PTM diagram for human tumour protein p63 (#TP63:p). The 'zines are pretty adamant that the protein is a Ub substrate of the anaphase promoting complex (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003458/). It has not been observed to be afflicted with SUMOylation. #proteomics #anapc
STXBP4 regulates APC/C-mediated p63 turnover and drives squamous cell carcinogenesis

The N-terminally truncated isoform of p63 (ΔNp63) is overexpressed in some forms of squamous cell carcinoma (SCC). Here we show that the anaphase-promoting complex/cyclosome (APC/C) degradation machinery plays an essential role in regulating the ...

PubMed Central (PMC)
Ron Beavis ❌Jan 24, 2024
α⧸ω ST phosphorylation diagrams for human & mouse cell divlsion cycle 20 (#CDC20:p). Both sequences have a dragon's neck type N-terminal phosphoIDR with three high occupancy S/TP acceptors. Phosphorylation is known to prevent association with the anaphase promoting complex. The cward shiny region is a kinase-resistant, WD40 repeat beta-propeller. #proteomics #anapc
Ron Beavis ❌Jan 23, 2024
A GPMDB ubiquitination PTM diagram for human Targeting Protein for Xenopus plus end-directed kinesin-like protein 2 (#TPX2:p). There are ubiquitination acceptors on this sequence that the 'zines suggest are substrates for anaphase promoting complex (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1291225/), it would appear that SUMOylation is doing some heavy lifting with this one, too. #proteomics #anapc
Anaphase-Promoting Complex/Cyclosome Controls the Stability of TPX2 during Mitotic Exit

TPX2, a microtubule-associated protein, is required downstream of Ran-GTP to induce spindle assembly. TPX2 activity appears to be tightly regulated during the cell cycle, and we report here one molecular mechanism for this regulation. We found that TPX2 ...

PubMed Central (PMC)
Ron Beavis ❌Jan 23, 2024
α⧸ω ST phosphorylation diagrams for human & mouse anaphase promoting complex subunit 10 (#ANAPC10:p). This subunit does not have any high occupancy phosphorylation acceptors, although both sequence have a low occupancy acceptor T7+phosphoryl. #proteomics #anapc
Show threadRon Beavis ❌Jan 22, 2024
A GPMDB ubiquitination PTM diagram for human polo like kinase 1 (#PLK1:p). This kinase is produced during G1. It is part of the mitosis coordination system that has be ubiquitylated by the anaphase promoting complex and removed for the process to proceed to completion. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467358). #proteomics #anapc
The role of Anaphase Promoting Complex activation, inhibition and substrates in cancer development and progression

The Anaphase Promoting Complex (APC), a multi-subunit ubiquitin ligase, facilitates mitotic and G1 progression, and is now recognized to play a role in maintaining genomic stability. Many APC substrates have been observed overexpressed in multiple cancer ...

PubMed Central (PMC)
Ron Beavis ❌Jan 22, 2024
α⧸ω ST phosphorylation diagrams for yeast anaphase promoting complex subunit 9 (#APC9:p). This subunit is present in yeast but there is no homolog in the mammalian complex. It has a single high occupancy receptor, T36+phosphoryl. #proteomics #anapc
Ron Beavis ❌Jan 21, 2024
A GPMDB ubiquitination PTM diagram for human aurora kinase B (#AURKB:p). Involved in microtubule coordination during mitosis, it has been identified as a Ub target of the anaphase-promoting complex (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467358). #proteomics #anapc
The role of Anaphase Promoting Complex activation, inhibition and substrates in cancer development and progression

The Anaphase Promoting Complex (APC), a multi-subunit ubiquitin ligase, facilitates mitotic and G1 progression, and is now recognized to play a role in maintaining genomic stability. Many APC substrates have been observed overexpressed in multiple cancer ...

PubMed Central (PMC)
Ron Beavis ❌Jan 21, 2024
α⧸ω ST phosphorylation diagrams for human & mouse cell division cycle 23 (#CDC23:p, aka #ANAPC8:p). Both sequences have multiple high occupancy S/TP-type acceptors on a short dragon's tail phosphoIDR. The nward bulk of the protein is composed of phosphorylation-resistant tetratricopeptide repeats. #proteomics #anapc
Ron Beavis ❌Jan 20, 2024
A GPMDB ubiquitination PTM diagram for human aurora kinase A (#AURKA:p). Involved in centrosome maturation & mitosis in general, it has been identified as a Ub target of the anaphase-promoting complex (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927175). #proteomics #anapc
Mechanisms for the temporal regulation of substrate ubiquitination by the anaphase-promoting complex/cyclosome

The anaphase-promoting complex/cyclosome (APC/C) is a multi-subunit, multifunctional ubiquitin ligase that controls the temporal degradation of numerous cell cycle regulatory proteins to direct the unidirectional cell cycle phases. Several different mechanisms ...

PubMed Central (PMC)
Ron Beavis ❌Jan 20, 2024
α⧸ω ST phosphorylation diagrams for human & mouse anaphase promoting complex subunit 7 (#ANAPC7:p). The human sequence has 2 low occupancy phosphoIDRs adjacent to the tetratricopeptide repeat helix bundles that dominate the shiny parts. #proteomics #anapc