Pipeline release! nf-core/ampliseq v2.16.1 - Ampliseq release 2.16.1 2026-01-23!
Amplicon sequencing analysis workflow using DADA2 and QIIME2
Please see the changelog: https://github.com/nf-core/ampliseq/releases/tag/2.16.1

#16s #18s #ampliconsequencing #edna #illumina #iontorrent #its #metabarcoding #metagenomics #metataxonomics #microbiome #pacbio #qiime2 #rrna #taxonomicclassification #taxonomicprofiling #nfcore #openscience #nextflow #bioinformatics

Release Ampliseq release 2.16.1 2026-01-23 · nf-core/ampliseq

Two bug fixes plus modifications to adhere to Nextflow strict syntax. Fixed #943 - Fix SciLifeLab Figshare urls for reference databases. #942 - Nextflow strict syntax for non-nf-core-modules/workf...

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Release Ampliseq Version 2.15.0 · nf-core/ampliseq

nf-core/ampliseq version 2.15.0 - 2025-10-06 Summary of changes Many issues were fixed and more control was given over QC and taxonomic classification. Detailed changes Added #909,#914 - Parameter...

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Release Ampliseq Version 2.14.0 · nf-core/ampliseq

nf-core/ampliseq version 2.14.0 - 2025-05-23 Summary of changes Multi-region analysis is now easier with custom databases, parameters were changed or added Updated sbdi-gtdb, gtdb, PR2 databases a...

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Release Ampliseq Version 2.13.0 · nf-core/ampliseq

nf-core/ampliseq version 2.13.0 - 2025-04-04 Summary of changes Zenodo changed from zenodo.org/record to zenodo.org/records and some taxonomic databases could not be downloaded any more Add most r...

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Release Ampliseq Version 2.12.0 · nf-core/ampliseq

nf-core/ampliseq version 2.12.0 - 2024-11-15 Summary of changes updated some reference databases several bug fixes updated to nf-core template 3.0.2 dependency updates Detailed changes Added #78...

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"rarefaction was actually the most robust approach for controlling for uneven sequencing effort across samples. Rarefaction limits the rate of falsely detecting and rejecting differences between treatment groups. Far from being "inadmissible", rarefaction is a valuable tool for analyzing microbiome sequence data."

Waste not, want not: Revisiting the analysis that called into question the practice of rarefaction

#metabarcoding #rarefaction #AmpliconSequencing

https://doi.org/10.1101/2023.06.23.546312