Hey yeast researchers,

Do you use frozen competent yeast cells for PCR-mediated integrations? What protocol do you use?

I'm thinking that this might save my lab a lot of time if they work reliably.

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Whoa!!! Some batches of agar are toxic to fission yesst!!

https://www.biorxiv.org/content/10.1101/2024.06.06.597796v1

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Sustainability tip for yeast labs:
when replica plating you can combine two different dissection plates into one replica plate. This will reduce the number of plates you use by almost ½.
Here’s how it works.

As you know, a normal dissection takes up an entire plate, and then to genotype you need to replica plate to many (5) different plates—each reporting on a different marker.

To me, that’s a lot of plates.

🧵 /1

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Does anyone in the US have the pOM plasmid set for N-terminal tagging in yeast made by Robert Gauss and Anne Spang? The last time I got strains from Anne they got hung up in customs for weeks and I'd like to get started soon!!

Any plasmid set for markerless N-terminal tagging would do.

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