Mastodawn

"Saline-free preparation for chronic in vivo imaging in adult Drosophila", Zhu et al. 2026 (Cheng Huang's lab)
https://www.biorxiv.org/content/10.64898/2026.02.18.706199v1

"We demonstrate the utility of this approach by tracking single-neuron spiking and neuronal calcium dynamics over 7–10 days."

"Longitudinal voltage imaging with single-spike resolution across 10 days ... We used the MBON05-specific split-GAL4 line SS01188 to express the voltage indicator pAce into the pair of MBON05 neurons."

#Drosophila #neuroscience #VoltageImaging

Saline-free preparation for chronic in vivo imaging in adult Drosophila

Longitudinal brain imaging is essential for understanding neural mechanisms. Here, we present a saline-free, chronic preparation for repeated neural recording in adult Drosophila over multiple days. We describe steps for mounting flies, performing manual surgery on the head cuticle without external saline, and resealing the opening to create a transparent optical window. We demonstrate the utility of this approach by tracking single-neuron spiking and neuronal calcium dynamics over 7–10 days. This protocol is potentially applicable to other insect species. ![Figure][1]</img> ### Competing Interest Statement The authors have declared no competing interest. Whitehall Foundation, https://ror.org/00her7k05, 2024-12-093 [1]: pending:yes

bioRxiv

Fabrizio Musacchio Jan 6

🧠 New preprint by Lee et al.: Fast dendritic excitations primarily mediate #backpropagation in #CA1 pyramidal #neurons during #behavior

Using kHz #VoltageImaging across the full #dendritic tree, they show that fast dendritic spikes are usually driven by somatic #bAPs, not independently initiated. #bAP propagation into apical dendrites is contin. modulated by pre-spike dendritic voltage & can trigger slower plateau potentials linked to complex spikes.

🌍https://doi.org/10.64898/2026.01.03.696606

#NeuralDynamics

Fabrizio Musacchio Oct 9, 2025

Great having Mark Schnitzer at today’s #iBehave lecture at the @dzne! Fascinating talk on cell-type specific #VoltageImaging 🧠⚡ of neural #spiking, #oscillations, #waves, and #MemoryDynamics. Showing that spike-resolution #imaging 🔬 in vivo is finally there 💪

#Neuroscience 🧪 #DZNE

Fabrizio Musacchio Oct 9, 2025

Great having Mark Schnitzer at today’s #iBehave lecture at the @dzne.science! Fascinating talk on cell-type specific #VoltageImaging 🧠⚡ of neural #spiking, #oscillations, #waves, and #MemoryDynamics. Showing that spike-resolution #imaging 🔬 in vivo is finally there 💪 #Neuroscience 🧪 #DZNE

Fabrizio Musacchio Sep 25, 2023

Promising new #imagedenoising tool: SUPPORT. Designed for #VoltageImaging and time lapse images in general:

🎥 https://static-content.springer.com/esm/art%3A10.1038%2Fs41592-023-02005-8/MediaObjects/41592_2023_2005_MOESM13_ESM.mp4 (demo)
✍️ Eom, M., Han, S., Park, P. et al. Statistically unbiased prediction enables accurate denoising of voltage imaging data. #NatMethods (2023). https://doi.org/10.1038/s41592-023-02005-8
👨‍💻 https://github.com/NICALab/SUPPORT

Fabrizio Musacchio Sep 13, 2023

#FIOLA, new pipeline for processing #CalciumImaging or #VoltageImaging 🌌🔬data, including fast speed and online processing 👌

✍️ Cai, C., Dong, C., Friedrich, J. et al. FIOLA: an accelerated pipeline for fluorescence imaging online analysis. #NatMethods
🌍 https://doi.org/10.1038/s41592-023-01964-2

#neuroscience