Hao YinKidney epithelial #Tubuloid
CD24+ epithelial progenitor-derived
4-phase protocol, Matrigel +Wnt3a/RSPO1, grow > 6 mo
CRISPR modeling #ADPKD, Cyst size responsive to #Tolvaptan
Super-impressive single-cell or single-nuclei RNAseq analyses to dissect the spatiotemporal molecular/cellular dynamics of this cool renal tubuloid, laying a critical foundation to enhance its clinical utilities👍
Wonderful #Lumenogenesis of CD24+ epithelial progenitor-derived nephrospheres, day 7 in Matrigel🤠
Dr. Rafael Kramann lab @NatureGenet 2022 @jackxuyx @KuppeChristoph @rkramann
https://www.nature.com/articles/s41588-022-01202-z
Adult human kidney organoids originate from CD24+ cells and represent an advanced model for adult polycystic kidney disease - Nature Genetics
Adult kidney organoids, or tubuloids, originate from CD24+ epithelial cells. Tubuloids represent a functional kidney tubule and can be used to model autosomal dominant polycystic kidney disease and study drug response.