RE: https://genomic.social/@PuckerLab/116746004467539229
If you are interested in free full plasmid sequencing, have a look at our preprint and get in touch:
https://doi.org/10.64898/2026.04.01.715842
#Plasmids #Cloning #Biotech #SynBio #iGEM #LongReads #Python #Bioinformatics
Please share with your network:
Would you benefit from full plasmid sequencing? Check out this cost-effective solution via nanopore long-read sequencing on old flow cells:
https://doi.org/10.64898/2026.04.01.715842
Long-read sequencing has shown a rapid technological development during the last years. It has been established as the standard method for the sequencing of plant genomes and has also gained importance for full plasmid sequencing. As Sanger sequencing has a limited read length of about 1 kb, long read sequencing offers a great advantage, as the full plasmid can be sequenced in one read. Here, we present a cost-effective workflow to sequence full plasmids and compare the results against an expectation. The per plasmid cost of this workflow is determined by the number of plasmids investigated simultaneously, but can be lower than the price of a single Sanger sequencing reaction. We developed a workflow for automatic data processing, which allows us to complete sequencing and data analysis within a day. ### Competing Interest Statement The authors have declared no competing interest.
We are looking for a new group member (BSc/MSc student) to bring our full plasmid sequencing data analysis workflow (NanoPlasmiQC) to the next level.
If you are excited about long-read sequencing and bioinformatics, this project could be for you and your thesis. Please find additional details here:
https://www.izmb.uni-bonn.de/en/pbb/news#NPQCS
More details about NanoPlasmiQC:
https://doi.org/10.64898/2026.04.01.715842
#Python #Genomics #LongReads #Biotech #SynBio #Bioinformatics
@PuckerLab
@samnm
This is awesome ...
Nigerian distributors made a home-made promotion video about the product they bought and are reselling.
https://www.youtube.com/shorts/EbWhJe-s8Bc
No AI, just a minimal-cost high effort video tailored to an audience and culture I know way too little about.
full disclosure: The product is something I worked on đ so I guarantee it works very well.
#dna #rna #dnasynthesis #molecularEngineering #molecularbiology #synbio #kilobaser #nigeria
Zhang et al. trace the history of #VitaminE-from its initial characterization and the discovery of its link to chlorophyll degradation to #sustainable microbial #manufacturing, highlighting innovative future #tech!
https://doi.org/10.1111/jipb.70235
@WileyLifeSci
#PlantSci #JIPB #SynBio
Would you like to confirm your cloning success with full plasmid sequencing?
NanoPlasmiQC allows automatic analysis of dozens of plasmids in a single ONT sequencing run. If you would like to test it with your own plasmids, read more in the preprint and feel free to reach out:
https://doi.org/10.64898/2026.04.01.715842
#Genomics #Bioinformatics #OpenScience #Biotechnology #SynBio #iGEM
@PuckerLab
Funded PhD position on novel crop pest and pathogen diagnostics available based at Fera Ltd. in collaboration with my lab - please share with any UK students who may be interested: https://generationresearch.ac.uk/mres-and-phd-studentships
Boas Pucker
PuckerLab
MrPhilSlade
xro
Nonya Bidniss
JIPB
Tom Howard
