ISR alone won’t cut it: stalled ribosomes block stress granule formation despite ISR activation.
Mohammed Jalloh highlights a #preprint from the Moon lab.
#preLight ⬇️ 👀
👉 https://prelights.biologists.com/highlights/trna-synthetase-activity-is-required-for-stress-granule-and-p-body-assembly/
#biology #stress #StressGranules #translation #biochemistry #ribosomes
Stalling the stress response: chronic low-level stress inhibits stress granule formation via translation elongation impairment.
New post from Mohammed Jalloh #UniversityofArizona highlighting work from the Ivanov lab. #preprint
#preLight ⬇️👀
https://prelights.biologists.com/highlights/chronic-stress-antagonizes-formation-of-stress-granules/
#biology #preprint #stress #StressGranules #translation #biochemistry
A very nice review on RNA granules from Geraldine Seydoux's lab at Genes & Development.
"RNA granules: functional compartments or incidental condensates?"
#RNA #granules #mRNA #phaseseparation #translation #splicing #Pbodies #StressGranules
#GermGranules
In EMBO Journal issue 3:
Host #mucins negotiate peace with #toxigenic #Vibrio
Cytosolic #stressgranules relieve nuclear #ubiquitin-#proteasome system
#Smoothened #cholesterylation and ER exit links in #Hedgehog signaling
Developmental #cellfate decision control by #chromatin reader #TRIM33
Cover by Kimon Lemonides, Helen Walden et al
LSI Paper of the Day☀️
Dr. Jibin Sadasivan and a team from the Eric Jan Lab provide new insights into how viruses manipulate the formation of stress granules - a potential strategy for protecting viral proteins and RNA and promoting infection in a new @PLOS Pathogens study.
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1010598#ack
Author summary Viruses often inhibit a cellular stress response that leads to the accumulation of RNA and protein condensates called stress granules. How this occurs and why this would benefit virus infection are not fully understood. Here, we reveal a viral protein that can block stress granules and identify a key amino acid residue in the protein that inactivates this function. We demonstrate that this viral protein has multiple functions to modulate nuclear events including mRNA export and transcription to regulate stress granule formation. We identify a key host protein that is important for viral protein-mediated stress granule inhibition, thus providing mechanistic insights. This study reveals a novel viral strategy in modulating stress granule formation to promote virus infection.
This paper looks interesting:
"An RNA granule for translation quality control in Saccharomyces cerevisiae"
#mRNA #granules #translation #StressGranules #NoGoDecay #RQC #yeast
Cytoplasmic RNA granules compartmentalize phases of the translation cycle in eukaryotes. We previously reported the localization of oxidized RNA to cytoplasmic foci called oxidized RNA bodies (ORBs) in human cells. We show here that ORBs are RNA granules in Saccharomyces cerevisiae. Several lines of evidence support a role of ORBs in the compartmentalization of no-go decay and ribosome quality control, the translation quality control pathways that recognize and clear aberrant mRNAs, including those with oxidized bases. Translation is required by these pathways and ORBs. Translation quality control factors localize to ORBs. A substrate of translation quality control, a stalled mRNA-ribosome-nascent chain complex, localizes to ORBS. Translation quality control mutants have altered ORB numbers, sizes, or both. In addition, we identify 68 ORB proteins, by immunofluorescence staining directed by proteomics, which further support their role in translation quality control and reveal candidate new factors for these pathways.
preLights
Gal Haimovich 😷🔬☣🏳️🌈🏳️⚧
The EMBO Journal
UBC Life Sciences Institute