SBGrid's eLife paper received a citation in May from Brandt Eichman at Vanderbilt University in EMBO Reports: DNA binding and lesion recognition by the bacterial interstrand DNA crosslink glycosylase AlkX.
Interstrand DNA crosslinks (ICLs) are a highly toxic form of DNA damage. ICL repair in both eukaryotes and bacteria involves unhooking of the two strands by specialized DNA glycosylases. We recently established that the human pathogen Acinetobacter baumannii contains an ICL glycosylase (AlkX) that facilitates pathogenesis and protects the bacteria from DNA damage and acid stress. However, the physical basis for glycosylase-catalyzed ICL unhooking is unknown. Here, we describe a crystal structure of AlkX bound to DNA representing a product of the ICL unhooking reaction. Mutational analysis of ICL unhooking in vitro and A. baumannii sensitivity to the crosslinking agent mechlorethamine enable the identification of several AlkX motifs critical for ICL repair. We also find that a genetic variant from an antibiotic-resistant strain of the human pathogen Salmonella enterica reduces AlkX activity in vitro and increases A. baumannii sensitivity to DNA crosslinking. This work provides a structural basis for how bacterial ICL glycosylases recognize and repair DNA adducts and contributes additional evidence that ICL repair is important for fitness of human pathogens.
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Sept 7-11, 2026 | Brno, Czechia
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Staying Connected to God
Have you ever had a friendship slowly drift apart? No argument. No betrayal. Just less communication. Less connection. Less time together. Our relationship with God can drift the same way if we're not careful. He reminds us that connection must happen on both ends - no matter how mush He calls, if we don't answer we will never connect. Click here to read the full devotional. ✨https://gemsofknowledge.com/2026/06/14/staying-connected-to-god/
SBGrid's eLife paper received a citation in May from Seth Darst at The Rockefeller University in Molecular Cell: Structural basis for multi-subunit DNA-dependent RNA polymerase catalytic activity.
Loneliness In Remote Workers Is Rising Here’s How To Combat Isolation When Working From Home
Loneliness Awareness Week is fast approaching and for many remote workers, isolation has become second nature. If you’re part of the work-from-home force and the loneliness has hit, here are our top tips to stay connected. For some people, working from home feels like a huge win: the flexibility, the work-life balance, the non-existent commute. But for others, being stuck at home alone all day, every day can feel like an isolating curse......Continue reading... By: Lily Smith Source: […]https://onlinemarketingscoops.com/2026/06/11/loneliness-in-remote-workers/
Loneliness Awareness Week is fast approaching and for many remote workers, isolation has become second nature. If you’re part of the work-from-home force and the loneliness has hit, here are our to…
SBGrid's eLife paper received a citation in April from Antonina Roll-Mecak from the The National Institutes of Health in Nature Structural and Molecular Biology: Microtubules in the axon are GDP bound but adopt a stable GTP-like expanded state.
Zehr et al. revealed the 2.7-Å cryo-electron microscopy reconstruction of human microtubules in situ in the axon of induced pluripotent stem cell (iPS cell)-derived neurons. It shows an expanded microtubule lattice yet bound to GDP, in contrast to the compacted lattice observed at the iPS cell stage.
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