I'm seeing disturbing patterns of behavior from #ONT #sequencing vendors.

Guys, no one cares about your flye/autocycler assembly pipeline & cookie cutter plots. Deliver at least fastq and in most cases pod5 to your customers. If you make that step difficult, your business is built on theft.

Academics, if a vendor is hesitant about offloading raw data to you ASAP (darn things are expensive to store), something's gone terribly wrong. Or the vendor thinks you're dumb, or both.

#microbiology

What I'm also doing is assessing 3rd party sequencing vendor ONT output quality using some variations of these tools. Feel free to get in touch if you have a sequencing vendor you want to either recommend or have assessed!

#sequencing #microbiology #archaea #ONT #nanopore #bioinformatics

I've been deep into ONT basecalling model land as part of my recent archaea study, trying to see if it's worth it in the first place.

Here's read to phred map plot per hour of sequencing time- ONT SUP model on the left, and archaea tuned custom model on the right. Differences are certainly present - though how it propagates downstream is still an open question.

#bioinformatics #ONT #sequencing #basecaller

After more than two weeks, the Grauniad now has an #obituary for #CraigVenter: https://www.theguardian.com/science/2026/may/14/craig-venter-obituary I was kind of surprised that he died at a normal age, rather than using his resources to engineer his own immortality.

#science #genome #DNA #sequencing #humanGenome

Speaking of - anyone know if there's any documentation/literature on how ONT put together their bacterial dorado polishing model?

I know it fares well in benchmarks, but curious about non-bacterial prokaryotes. Is anyone putting together their own polishing models for test?

#bioinformatics #sequencing #ONT

Single-molecule #peptide #sequencing through reverse translation of peptides into DNA
https://www.nature.com/articles/s41587-026-03061-z

everything that can be bound with/converted to DNA can be "sequenced": it's sort of like SBS, but here DNA oligos are used instead of individual DNA bases and later actual SBS is performed to decode each oligo sequence... every read will inform about single aa at single position from single protein.
to sequence a protein with 300 aa, you'll need to sequence at least 300 reads!
neat!

RE: https://genomic.social/@jfy133/116091864495660495

🚨 LAST CHANCE! 🚨

🧬 Do you work with #sequencing data, and have a spare 5 minutes to help contribute to making #genomics #metadata standards more #FAIR?|

🎰 Want a chance to win a little prize pack?

💡 Reminder of the our @NFDI4Microbiota and Genomic Standards Consortium funded survey (link below)!

📅 Deadline is 20th March (end of tomorrow!)!